Interaction with fibronectin regulates cytokine gene expression in human melanoma cells

Raffaella Lupetti, Roberta Mortarini, Pierangelo Panceri, Marialuisa Sensi, Andrea Anichini

Research output: Contribution to journalArticle

Abstract

Our study was aimed at investigating whether interaction of human melanoma cells with the extracellular matrix (ECM) protein fibronectin (FN) could regulate lymphokine gene expression. Serum-deprived cells (quiescent condition) of a metastatic melanoma cloned line were cultured either on uncoated or on FN- or BSA-coated surfaces. By means of reverse transcriptase-polymerase chain reaction (RT-PCR), we analyzed mRNA expression of 4 cytokines - interleukin (IL)-Iα, IL-1β, IL-6 and IL-8 - and 9 growth factors - endothelial cell growth factor (ECGF), basic fibroblast growth factor (bFGF), fibroblast growth factor (FGF)-5, HST, keratinocyte growth factor (KGF), transforming growth factor (TGF)-α, TGF-β1, TGF-β2 and TGF-β3. When cultured on FN, melanoma cells expressed IL-1α and IL-6 transcripts in addition to IL-1β, IL-8, ECGF, TGF-β1, TGF-β2 and TGF-β3, already present in quiescent cells, Amplification parameters to achieve semi-quantitative RT-PCR were then determined for each detectable factor, thus allowing us to measure a selective enhancement of mRNA levels for IL-1α, IL-6, IL-8 and TGF-β2 upon interaction with FN by quiescent melanoma cells. This augmented expression was inhibited by an anti-integrin pi chain monoclonal antibody (MAb). Moreover, the amounts of IL-6, IL-8 and IL-β produced in the supernatants, as assessed by ELISA, correlated with the corresponding mRNA expression. Extension of this analysis to the other 5 human primary and metastatic melanoma lines confirmed the ability of FN to selectively up-regulate only IL-6 and IL-8 secretion. Our data indicate that FN is able to modulate expression and secretion of a defined subset of lymphokines in human melanoma.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalInternational Journal of Cancer
Volume66
Issue number1
DOIs
Publication statusPublished - Mar 28 1996

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Transforming Growth Factors
Fibronectins
Melanoma
Cytokines
Gene Expression
Interleukin-8
Interleukin-1
Interleukin-6
Endothelial Growth Factors
Lymphokines
Reverse Transcriptase Polymerase Chain Reaction
Messenger RNA
Fibroblast Growth Factor 5
Endothelial Cells
Interleukin-9
Fibroblast Growth Factor 7
Extracellular Matrix Proteins
Interleukins
Fibroblast Growth Factor 2
Integrins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Interaction with fibronectin regulates cytokine gene expression in human melanoma cells. / Lupetti, Raffaella; Mortarini, Roberta; Panceri, Pierangelo; Sensi, Marialuisa; Anichini, Andrea.

In: International Journal of Cancer, Vol. 66, No. 1, 28.03.1996, p. 110-116.

Research output: Contribution to journalArticle

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