Interaction with fibronectin regulates cytokine gene expression in human melanoma cells

Raffaella Lupetti, Roberta Mortarini, Pierangelo Panceri, Marialuisa Sensi, Andrea Anichini

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Our study was aimed at investigating whether interaction of human melanoma cells with the extracellular matrix (ECM) protein fibronectin (FN) could regulate lymphokine gene expression. Serum-deprived cells (quiescent condition) of a metastatic melanoma cloned line were cultured either on uncoated or on FN- or BSA-coated surfaces. By means of reverse transcriptase-polymerase chain reaction (RT-PCR), we analyzed mRNA expression of 4 cytokines - interleukin (IL)-Iα, IL-1β, IL-6 and IL-8 - and 9 growth factors - endothelial cell growth factor (ECGF), basic fibroblast growth factor (bFGF), fibroblast growth factor (FGF)-5, HST, keratinocyte growth factor (KGF), transforming growth factor (TGF)-α, TGF-β1, TGF-β2 and TGF-β3. When cultured on FN, melanoma cells expressed IL-1α and IL-6 transcripts in addition to IL-1β, IL-8, ECGF, TGF-β1, TGF-β2 and TGF-β3, already present in quiescent cells, Amplification parameters to achieve semi-quantitative RT-PCR were then determined for each detectable factor, thus allowing us to measure a selective enhancement of mRNA levels for IL-1α, IL-6, IL-8 and TGF-β2 upon interaction with FN by quiescent melanoma cells. This augmented expression was inhibited by an anti-integrin pi chain monoclonal antibody (MAb). Moreover, the amounts of IL-6, IL-8 and IL-β produced in the supernatants, as assessed by ELISA, correlated with the corresponding mRNA expression. Extension of this analysis to the other 5 human primary and metastatic melanoma lines confirmed the ability of FN to selectively up-regulate only IL-6 and IL-8 secretion. Our data indicate that FN is able to modulate expression and secretion of a defined subset of lymphokines in human melanoma.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalInternational Journal of Cancer
Volume66
Issue number1
DOIs
Publication statusPublished - Mar 28 1996

Fingerprint

Transforming Growth Factors
Fibronectins
Melanoma
Cytokines
Gene Expression
Interleukin-8
Interleukin-1
Interleukin-6
Endothelial Growth Factors
Lymphokines
Reverse Transcriptase Polymerase Chain Reaction
Messenger RNA
Fibroblast Growth Factor 5
Endothelial Cells
Interleukin-9
Fibroblast Growth Factor 7
Extracellular Matrix Proteins
Interleukins
Fibroblast Growth Factor 2
Integrins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Interaction with fibronectin regulates cytokine gene expression in human melanoma cells. / Lupetti, Raffaella; Mortarini, Roberta; Panceri, Pierangelo; Sensi, Marialuisa; Anichini, Andrea.

In: International Journal of Cancer, Vol. 66, No. 1, 28.03.1996, p. 110-116.

Research output: Contribution to journalArticle

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