Interference of dibutylphthalate on human prostate cell viability

Mariana Di Lorenzo, Maurizio Forte, Salvatore Valiante, Vincenza Laforgia, Maria De Falco

Research output: Contribution to journalArticle

Abstract

Dibutylphthalate (DBP) is an environmental pollutant widely used as plasticizer in a variety of industrial applications worldwide. This agent can be found in personal-care products, children's toy, pharmaceuticals, food products. Exposure to DBP can occur via ingestion and inhalation as well as intravenous or skin contact. DBP belongs to the family of endocrine disrupting chemicals (EDCs) and its effects on reproductive system were demonstrated both in vivo and in vitro. In the present study we evaluated the effects of DBP on human prostate adenocarcinoma epithelial cells (LNCaP) in order to highlight xenoestrogens influence on human prostate. Moreover, we have compared DBP effects with 17β-estradiol action in order to investigate possible mimetical behaviour. We have assessed the effects of both compounds on the cell viability. After then, we have evaluated the expression of genes and proteins involved in cell cycle regulation. Furthermore, we have observed the expression and the cell localization of estrogen (ERs) and androgen (AR) receptors. In conclusion, we have demonstrated that DBP interacts with estrogen hormonal receptor pathway but differently from E2. DBP alters the normal gland physiology and it is involved in the deregulation of prostate cell cycle.

Original languageEnglish
Pages (from-to)565-573
Number of pages9
JournalEcotoxicology and Environmental Safety
Volume147
DOIs
Publication statusPublished - Jan 1 2018

Keywords

  • Dibutylphthalate
  • EDCs
  • Estrogens
  • Phthalate
  • Prostate
  • Xenoestrogens

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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