Interferon-γ activated calcium influx in peripheral blood lymphocytes from patients with primary and secondary progressive multiple sclerosis

Gianvito Martino, Elena Brambilla, Massimo Filippi, Vittorio Martinelli, Bruno Colombo, Mariaemma Rodegher, Giancarlo Comi, L. M E Grimaldi

Research output: Contribution to journalArticle

Abstract

Interferon-γ (IFN-γ contributes to the early events leading to T cell. activation in relapsing-remitting (RR) multiple sclerosis (MS) by activating a transplasmalemma calcium influx, the detection of which is closely associated with clinical and MRI evidence of disease activity. The appearance of this influx represents one of the earliest peripheral events in the pathogenesis of RRMS. It is still questioned whether the same immune mediated mechanisms also operate in primary progressive (PP)MS. Fluorimetric evidence of the IFN-γ activated calcium influx was sought in 16 patients with PPMS and 39 patients with secondary progressive (SP)MS. To compare peripheral versus CNS evidence of immune activation 11 of the patients with PPMS and 27 of the patients with SPMS underwent gadolinium enhanced brain MRI. The IFN-γ activated influx was detected in peripheral blood lymphocytes from eight of 16 (50%) patients with PPMS, and 20 of 39 (51%) patients with SPMS, a frequency similar to that previously reported in patients with RRMS during phases of disease stability. Gadolinium enhancing brain MRI lesions were found in only one of 11 (9%) patients with PPMS and 12 of 27 (41%) with SPMS. Our study shows that peripheral blood lymphocytes from patients with PPMS and patients with SPMS express with the same frequency as patients with RRMS, an IFN-γ dependent intracellular process leading to T cell activation able to trigger disease activity.

Original languageEnglish
Pages (from-to)515-517
Number of pages3
JournalJournal of Neurology, Neurosurgery and Psychiatry
Volume61
Issue number5
Publication statusPublished - Nov 1996

Keywords

  • Calcium
  • Interferon-γ
  • Primary progressive multiple sclerosis
  • T lymphocytes

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology

Fingerprint Dive into the research topics of 'Interferon-γ activated calcium influx in peripheral blood lymphocytes from patients with primary and secondary progressive multiple sclerosis'. Together they form a unique fingerprint.

  • Cite this