Abstract
Pro- and anti-inflammatory cytokines are thought to participate in the development and regulation of autoimmunity in multiple sclerosis (MS), a demyelinating disease of the central nervous system (CNS). We analysed the percentage of interferon (IFN)-γ and interleukin (IL)-4-producing cells in the peripheral blood of both active and stable MS patients, and of healthy controls. After short-term stimulation, cytokine-producing cells were intracellularly stained and sorted. Significantly lower percentages of IFN-γ and IL-4-producing T cells were found in stable MS patients than in controls, and in active than in stable patients. The diminution affected CD4+ (Th1, Th2) and CD8+ (Tc1) phenotypes. Tc2 cells were not detected. The Th1/Th2 ratio did not differ in active and stable MS, nor in controls. The fact that Th2 and Tc1 cell percentages were higher in stable than in active MS possibly indicates that these cells play a downmodulating role in the immune response. In contrast, a role in exacerbating the immune response is not attributable to Th1 cells, given their reduction in acute MS. Our data do not support the hypothesis that MS is a Th1/Th2 paradigmatic disease; rather, they suggest that sequestration in the CNS, or activation-induced apoptosis (whether in vivo or in vitro) may explain reduced levels of IFN-γ and IL-4-producing subsets in the peripheral blood of clinically acute patients.
| Original language | English |
|---|---|
| Pages (from-to) | 677-681 |
| Number of pages | 5 |
| Journal | European Cytokine Network |
| Volume | 11 |
| Issue number | 4 |
| Publication status | Published - 2000 |
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Keywords
- Flow cytometry
- IFN-γ
- IL-4
- Multiple sclerosis
- Tc1/Tc2 cells
- Th1/Th2 cells
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
Interferon-γ and interleukin-4-producing T cells in peripheral blood of multiple sclerosis patients. / Franciotta, D.; Zardini, E.; Bergamaschi, R.; Andreoni, L.; Cosi, V.
In: European Cytokine Network, Vol. 11, No. 4, 2000, p. 677-681.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interferon-γ and interleukin-4-producing T cells in peripheral blood of multiple sclerosis patients
AU - Franciotta, D.
AU - Zardini, E.
AU - Bergamaschi, R.
AU - Andreoni, L.
AU - Cosi, V.
PY - 2000
Y1 - 2000
N2 - Pro- and anti-inflammatory cytokines are thought to participate in the development and regulation of autoimmunity in multiple sclerosis (MS), a demyelinating disease of the central nervous system (CNS). We analysed the percentage of interferon (IFN)-γ and interleukin (IL)-4-producing cells in the peripheral blood of both active and stable MS patients, and of healthy controls. After short-term stimulation, cytokine-producing cells were intracellularly stained and sorted. Significantly lower percentages of IFN-γ and IL-4-producing T cells were found in stable MS patients than in controls, and in active than in stable patients. The diminution affected CD4+ (Th1, Th2) and CD8+ (Tc1) phenotypes. Tc2 cells were not detected. The Th1/Th2 ratio did not differ in active and stable MS, nor in controls. The fact that Th2 and Tc1 cell percentages were higher in stable than in active MS possibly indicates that these cells play a downmodulating role in the immune response. In contrast, a role in exacerbating the immune response is not attributable to Th1 cells, given their reduction in acute MS. Our data do not support the hypothesis that MS is a Th1/Th2 paradigmatic disease; rather, they suggest that sequestration in the CNS, or activation-induced apoptosis (whether in vivo or in vitro) may explain reduced levels of IFN-γ and IL-4-producing subsets in the peripheral blood of clinically acute patients.
AB - Pro- and anti-inflammatory cytokines are thought to participate in the development and regulation of autoimmunity in multiple sclerosis (MS), a demyelinating disease of the central nervous system (CNS). We analysed the percentage of interferon (IFN)-γ and interleukin (IL)-4-producing cells in the peripheral blood of both active and stable MS patients, and of healthy controls. After short-term stimulation, cytokine-producing cells were intracellularly stained and sorted. Significantly lower percentages of IFN-γ and IL-4-producing T cells were found in stable MS patients than in controls, and in active than in stable patients. The diminution affected CD4+ (Th1, Th2) and CD8+ (Tc1) phenotypes. Tc2 cells were not detected. The Th1/Th2 ratio did not differ in active and stable MS, nor in controls. The fact that Th2 and Tc1 cell percentages were higher in stable than in active MS possibly indicates that these cells play a downmodulating role in the immune response. In contrast, a role in exacerbating the immune response is not attributable to Th1 cells, given their reduction in acute MS. Our data do not support the hypothesis that MS is a Th1/Th2 paradigmatic disease; rather, they suggest that sequestration in the CNS, or activation-induced apoptosis (whether in vivo or in vitro) may explain reduced levels of IFN-γ and IL-4-producing subsets in the peripheral blood of clinically acute patients.
KW - Flow cytometry
KW - IFN-γ
KW - IL-4
KW - Multiple sclerosis
KW - Tc1/Tc2 cells
KW - Th1/Th2 cells
UR - http://www.scopus.com/inward/record.url?scp=0034517626&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034517626&partnerID=8YFLogxK
M3 - Article
C2 - 11125313
AN - SCOPUS:0034517626
VL - 11
SP - 677
EP - 681
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
SN - 0893-6692
IS - 4
ER -