Interferon-γ increases expression of chemokine receptors CCR1, CCR3, and CCR5, but not CXCR4 in monocytoid U937 cells

Davide Zella, Oxana Barabitskaja, Jennifer M. Burns, Fabio Romerio, Daniel E. Dunn, Maria Grazia Revello, Giuseppe Gerna, Marvin S. Reitz, Robert C. Gallo, Frank F. Weichold

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Abstract

Chemokine receptors (CR), which can mediate migration of immune cells to the site of inflammation, also function as coreceptors for human immunodeficiency virus (HIV) entry into CD4+ T lymphocytes and antigen- presenting cells. We demonstrate here that interferon-γ (IFN-γ) increases the expression of chemokine receptors CCR1, CCR3, and CCR5 in monocytoid U937 cells as detected by cell surface molecule labeling and mRNA expression, as well as by intracellular calcium mobilization and cell migration in response to specific ligands. The increased expression of these chemokine receptors also results in an enhanced HIV-1 entry into cells. Our data provide evidence for a relationship of cellular pathways that are induced by IFN-γ with those that regulate chemokine receptor expression.

Original languageEnglish
Pages (from-to)4444-4450
Number of pages7
JournalBlood
Volume91
Issue number12
Publication statusPublished - 1998

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ASJC Scopus subject areas

  • Hematology

Cite this

Zella, D., Barabitskaja, O., Burns, J. M., Romerio, F., Dunn, D. E., Revello, M. G., Gerna, G., Reitz, M. S., Gallo, R. C., & Weichold, F. F. (1998). Interferon-γ increases expression of chemokine receptors CCR1, CCR3, and CCR5, but not CXCR4 in monocytoid U937 cells. Blood, 91(12), 4444-4450.