Interferon-γ receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death

Paola Bernabei, Eliana M. Coccia, Laura Rigamonti, Marita Bosticardo, Guido Forni, Sidney Pestka, Christopher D. Krause, Angela Battistini, Francesco Novelli

Research output: Contribution to journalArticle

Abstract

The heterodimeric interferon (IFN)-γ receptor (IFN-γR) is formed of two chains. Here we show that the binding chain (IFN-γR1) was highly expressed on the membranes of T, B, and myeloid cells. Conversely, the transducing chain (IFN-γR2) was highly expressed on the surfaces of myeloid cells, moderately expressed on B cells, and poorly expressed on the surfaces of T cells. Differential cell membrane expression of IFN-γR2 determined the number of receptor complexes that transduced the IFN-γ signal and resulted in a different response to IFN-γ. After IFN-γ stimulation, high IFN-γR2 membrane expression induced rapid activation of signal transducer and activator of transcription-1 (STAT-1) and high levels of interferon regulatory factor-1 (IRF-1), which then triggered the apoptotic program. By contrast, low cell membrane expression resulted in slow activation of STAT-1, lower levels of IRF-1, and induction of proliferation. Because the forced expression of IFN-γR2 on T cells switched their response to IFN-γ from proliferative to apoptotic, we concluded that the surface expression of IFN-γR2 determines whether a cell stimulated by IFN-γ undergoes proliferation or apoptosis.

Original languageEnglish
Pages (from-to)950-960
Number of pages11
JournalJournal of Leukocyte Biology
Volume70
Issue number6
Publication statusPublished - 2001

Fingerprint

Interferon Receptors
Myeloid Cells
Interferons
B-Lymphocytes
Cell Death
Cell Proliferation
T-Lymphocytes
Interferon Regulatory Factor-1
STAT1 Transcription Factor
Cell Membrane
Membranes

Keywords

  • Apoptosis
  • Human
  • IFN-γ
  • IFN-γ receptor
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology

Cite this

Bernabei, P., Coccia, E. M., Rigamonti, L., Bosticardo, M., Forni, G., Pestka, S., ... Novelli, F. (2001). Interferon-γ receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death. Journal of Leukocyte Biology, 70(6), 950-960.

Interferon-γ receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death. / Bernabei, Paola; Coccia, Eliana M.; Rigamonti, Laura; Bosticardo, Marita; Forni, Guido; Pestka, Sidney; Krause, Christopher D.; Battistini, Angela; Novelli, Francesco.

In: Journal of Leukocyte Biology, Vol. 70, No. 6, 2001, p. 950-960.

Research output: Contribution to journalArticle

Bernabei, P, Coccia, EM, Rigamonti, L, Bosticardo, M, Forni, G, Pestka, S, Krause, CD, Battistini, A & Novelli, F 2001, 'Interferon-γ receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death', Journal of Leukocyte Biology, vol. 70, no. 6, pp. 950-960.
Bernabei, Paola ; Coccia, Eliana M. ; Rigamonti, Laura ; Bosticardo, Marita ; Forni, Guido ; Pestka, Sidney ; Krause, Christopher D. ; Battistini, Angela ; Novelli, Francesco. / Interferon-γ receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death. In: Journal of Leukocyte Biology. 2001 ; Vol. 70, No. 6. pp. 950-960.
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