TY - JOUR
T1 - Interferon-γ-stimulated human keratinocytes express the genes necessary for the production of peptide-loaded MHC class II molecules
AU - Albanesi, C.
AU - Cavani, A.
AU - Girolomoni, G.
PY - 1998
Y1 - 1998
N2 - Keratinocytes exposed to interferon (IFN)-γ synthesize major histocompatibility complex class II antigens both in vivo and in vitro; however, the expression of class II accessory genes has not yet been investigated. In this study, we examined the capacity of normal human keratinocytes activated with IFN-γ to express HLA-DR, HLA-DM, and invariant chain genes as well as two major transcription regulatory genes, class II transactivator and RFX5. Cultured keratinocytes were shown to synthesize low levels of DMα, invariant chain p33, and RFX5 transcription factor. Upon treatment with IFN-γ, expression of RFX5, DMα, and invariant chain p33 mRNA increased, whereas class II transactivator mRNA appeared de novo, followed by the expression of DRα, DMβ, and invariant chain p41 genes. Western blot analysis showed the both p33 and p41 invariant chain forms and DM became detectable in keratinocytes after stimulation with IFN-γ, with a higher p41/p33 ratio compared with Raji B cells. Finally, HLA-DR molecules present on IFN-α-treated keratinocytes were shown to be remarkably resistant to sodium dodecyl sulfate denaturation at room temperature, a feature that class II molecules acquire when their groove is properly loaded with peptide. These results suggest that human keratinocytes activated with IFN-γ posses the biochemical requirements activated with IFN-γ possess the biochemical requirements for the generation of functional class II peptide complexes.
AB - Keratinocytes exposed to interferon (IFN)-γ synthesize major histocompatibility complex class II antigens both in vivo and in vitro; however, the expression of class II accessory genes has not yet been investigated. In this study, we examined the capacity of normal human keratinocytes activated with IFN-γ to express HLA-DR, HLA-DM, and invariant chain genes as well as two major transcription regulatory genes, class II transactivator and RFX5. Cultured keratinocytes were shown to synthesize low levels of DMα, invariant chain p33, and RFX5 transcription factor. Upon treatment with IFN-γ, expression of RFX5, DMα, and invariant chain p33 mRNA increased, whereas class II transactivator mRNA appeared de novo, followed by the expression of DRα, DMβ, and invariant chain p41 genes. Western blot analysis showed the both p33 and p41 invariant chain forms and DM became detectable in keratinocytes after stimulation with IFN-γ, with a higher p41/p33 ratio compared with Raji B cells. Finally, HLA-DR molecules present on IFN-α-treated keratinocytes were shown to be remarkably resistant to sodium dodecyl sulfate denaturation at room temperature, a feature that class II molecules acquire when their groove is properly loaded with peptide. These results suggest that human keratinocytes activated with IFN-γ posses the biochemical requirements activated with IFN-γ possess the biochemical requirements for the generation of functional class II peptide complexes.
KW - Antigen processing
KW - Epithelial cells
KW - HLA-DM
KW - Invariant chain
KW - MHC class II
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U2 - 10.1046/j.1523-1747.1998.00098.x
DO - 10.1046/j.1523-1747.1998.00098.x
M3 - Article
C2 - 9457908
AN - SCOPUS:0031907044
VL - 110
SP - 138
EP - 142
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 2
ER -