Interferon induction by HIV-1-infected cells: A possible role of sulfatides or related glycolipids

Helmut Ankel; Maria R. Capobianchi; Fabiola Frezza; Concetta Castilletti; Ferdinando Dianzani

doi:10.1006/viro.1996.0357

Interferon induction by HIV-1-infected cells: A possible role of sulfatides or related glycolipids

Helmut Ankel, Maria R. Capobianchi, Fabiola Frezza, Concetta Castilletti, Ferdinando Dianzani

Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani

Research output: Contribution to journal › Article › peer-review

Abstract

We have investigated the mechanism of interferon (IFN) induction in peripheral blood mononuclear cells by HIV-1(IIIB)-infected H9 cells or by recombinant gp120. A monoclonal antibody specific for the galactosylsphingosinyl moiety in galactocerebrosides and sulfatides inhibited IFN induction in a dose-dependent manner. Furthermore, exogenous sulfatides inhibited with an ID₅₀ of approximately 1 μM, whereas galactocerebrosides were not inhibitory at 40 times higher concentrations. These studies suggest that sulfate containing galactolipids such as sulfatides on responder cells may be part of the gp120-membrane complex that initiates the induction of IFN. A partial homology of an epitope on the V3 loop of gp120 with a previously suggested binding domain for sulfated glycoconjugates supports this conclusion.

Original language	English
Pages (from-to)	113-119
Number of pages	7
Journal	Virology
Volume	221
Issue number	1
DOIs	https://doi.org/10.1006/viro.1996.0357
Publication status	Published - Jul 1 1996

ASJC Scopus subject areas

Virology
Infectious Diseases

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abstract = "We have investigated the mechanism of interferon (IFN) induction in peripheral blood mononuclear cells by HIV-1(IIIB)-infected H9 cells or by recombinant gp120. A monoclonal antibody specific for the galactosylsphingosinyl moiety in galactocerebrosides and sulfatides inhibited IFN induction in a dose-dependent manner. Furthermore, exogenous sulfatides inhibited with an ID50 of approximately 1 μM, whereas galactocerebrosides were not inhibitory at 40 times higher concentrations. These studies suggest that sulfate containing galactolipids such as sulfatides on responder cells may be part of the gp120-membrane complex that initiates the induction of IFN. A partial homology of an epitope on the V3 loop of gp120 with a previously suggested binding domain for sulfated glycoconjugates supports this conclusion.",

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AU - Dianzani, Ferdinando

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