TY - JOUR
T1 - Interferon induction by HIV-1-infected cells
T2 - A possible role of sulfatides or related glycolipids
AU - Ankel, Helmut
AU - Capobianchi, Maria R.
AU - Frezza, Fabiola
AU - Castilletti, Concetta
AU - Dianzani, Ferdinando
PY - 1996/7/1
Y1 - 1996/7/1
N2 - We have investigated the mechanism of interferon (IFN) induction in peripheral blood mononuclear cells by HIV-1(IIIB)-infected H9 cells or by recombinant gp120. A monoclonal antibody specific for the galactosylsphingosinyl moiety in galactocerebrosides and sulfatides inhibited IFN induction in a dose-dependent manner. Furthermore, exogenous sulfatides inhibited with an ID50 of approximately 1 μM, whereas galactocerebrosides were not inhibitory at 40 times higher concentrations. These studies suggest that sulfate containing galactolipids such as sulfatides on responder cells may be part of the gp120-membrane complex that initiates the induction of IFN. A partial homology of an epitope on the V3 loop of gp120 with a previously suggested binding domain for sulfated glycoconjugates supports this conclusion.
AB - We have investigated the mechanism of interferon (IFN) induction in peripheral blood mononuclear cells by HIV-1(IIIB)-infected H9 cells or by recombinant gp120. A monoclonal antibody specific for the galactosylsphingosinyl moiety in galactocerebrosides and sulfatides inhibited IFN induction in a dose-dependent manner. Furthermore, exogenous sulfatides inhibited with an ID50 of approximately 1 μM, whereas galactocerebrosides were not inhibitory at 40 times higher concentrations. These studies suggest that sulfate containing galactolipids such as sulfatides on responder cells may be part of the gp120-membrane complex that initiates the induction of IFN. A partial homology of an epitope on the V3 loop of gp120 with a previously suggested binding domain for sulfated glycoconjugates supports this conclusion.
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U2 - 10.1006/viro.1996.0357
DO - 10.1006/viro.1996.0357
M3 - Article
C2 - 8661419
AN - SCOPUS:0030198851
VL - 221
SP - 113
EP - 119
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -