Interferon induction by HIV-1-infected cells: A possible role of sulfatides or related glycolipids

Helmut Ankel, Maria R. Capobianchi, Fabiola Frezza, Concetta Castilletti, Ferdinando Dianzani

Research output: Contribution to journalArticle

Abstract

We have investigated the mechanism of interferon (IFN) induction in peripheral blood mononuclear cells by HIV-1(IIIB)-infected H9 cells or by recombinant gp120. A monoclonal antibody specific for the galactosylsphingosinyl moiety in galactocerebrosides and sulfatides inhibited IFN induction in a dose-dependent manner. Furthermore, exogenous sulfatides inhibited with an ID50 of approximately 1 μM, whereas galactocerebrosides were not inhibitory at 40 times higher concentrations. These studies suggest that sulfate containing galactolipids such as sulfatides on responder cells may be part of the gp120-membrane complex that initiates the induction of IFN. A partial homology of an epitope on the V3 loop of gp120 with a previously suggested binding domain for sulfated glycoconjugates supports this conclusion.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalVirology
Volume221
Issue number1
DOIs
Publication statusPublished - Jul 1 1996

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ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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