Interferon-mediated enhancement of metastasis. Are MHC antigens involved?

Pier Luigi Lollini, Carla De Giovanni, Brunella Del Re, Giordano Nicoletti, Giorgio Prodi, Patrizia Nanni

Research output: Contribution to journalArticlepeer-review

Abstract

The relationship between major histocompatibility complex (MHC) antigens and metastasis was investigated on B16 melanoma variants. B16 cell lines express low amounts of murine MHC (H-2) antigens. A high expression can be induced in line B16-A by in vitro treatment with immune interferon (IFN-gamma) or by in vivo transplant in allogeneic mice. The increase of H-2 antigens correlated with an enhancement of lung colonization in young syngeneic mice. The higher metastatic capacity of B16-A cells with induced high levels of H-2 antigens was observed also in adult mice and in young mice pretreated with cyclophosphamide. These results were confirmed investigating the behaviour of a mutant B16 clone (B78H1) which was selectively resistant to the H-2-inducing action of IFN-gamma: lung colonization ability was not increased by IFN pretreatment. The study of variants derived from individual B16-A lung colonies revealed a wide range of H-2 levels. Variants with a low expression had a low colonization ability; one out of two variants with a high H-2 expression also was poorly colonizing. IFN-gamma-mediated H-2 expression appeared to act as an enhancer, rather than a determinant of B16 metastatic capacity.

Original languageEnglish
Pages (from-to)277-287
Number of pages11
JournalClinical & Experimental Metastasis
Volume5
Issue number4
DOIs
Publication statusPublished - Dec 1987

ASJC Scopus subject areas

  • Cancer Research

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