Interlaboratory evaluation of the genotoxic properties of pencycuron, a commonly used phenylurea fungicide

Károly Nagy, Congying Zheng, Claudia Bolognesi, Balázs Ádám

Research output: Contribution to journalArticle

Abstract

Pencycuron, a phenylurea-type antifungal agent, is used in agriculture worldwide for inhibiting the growth of various fungal pathogens of crops. Pencycuron residues were found in vegetables, soil and drinking water. Accordingly, both occupational and consumer exposure can be expected and may be significant. However, human toxicity studies on its genotoxic, mutagenic or carcinogenic potential are lacking. Therefore, a collaborative study was performed in two laboratories to investigate whether pencycuron exposure can induce DNA damage. The genotoxic effect of 0–100 μg/ml pencycuron in in vitro cultures of human mononuclear white blood cells (MWBCs) and human hepatocytes (HepG2) was detected by cytokinesis-block micronucleus assay and comet assay. The combined results of the two labs showed a dose-dependent DNA damage detected by micronucleus frequency, which reached statistical significance at 100 μg/ml concentration after 21-h exposure in HepG2 cells (p = 0.048). Significant genotoxic effect could also be observed in the comet assay from 50 μg/ml concentration in MWBCs, and at 100 μg/ml concentration in HepG2 cells in one lab. Nevertheless, this finding was not confirmed by the other lab in HepG2 cells, where Fpg-dependent oxidative DNA damage could also not be detected. The results indicate that pencycuron may have DNA-damaging potential as well as point out inter-laboratory variability that calls for further studies to confirm the genotoxicity of this fungicide.

LanguageEnglish
Pages1052-1057
Number of pages6
JournalScience of the Total Environment
Volume647
DOIs
Publication statusPublished - Jan 10 2019

Fingerprint

Fungicides
fungicide
DNA
Assays
assay
comet
damage
Blood
Antifungal agents
blood
genotoxicity
Vegetables
Pathogens
Cell culture
Potable water
Agriculture
Antifungal Agents
vegetable
Crops
Toxicity

Keywords

  • Comet assay
  • DNA damage
  • Fungicide
  • Genotoxicity
  • Micronucleus test
  • Pencycuron
  • Pesticide

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Interlaboratory evaluation of the genotoxic properties of pencycuron, a commonly used phenylurea fungicide. / Nagy, Károly; Zheng, Congying; Bolognesi, Claudia; Ádám, Balázs.

In: Science of the Total Environment, Vol. 647, 10.01.2019, p. 1052-1057.

Research output: Contribution to journalArticle

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