Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material

Françoise Schiele, Jocelyne Muller, Elisabeth Colinet, Gérard Siest, P. Arzoglou, H. Brettschneider, D. H. Calam, Ferruccio Ceriotti, Georges Férard, Jörg Frei, A. Hadjivassiliou, J. C M Hafkenscheid, Mogens Hørder, F. Javier Gella, Robert Rej, Ellen Schmidt, Älnne Vassault, V. Viette

Research output: Contribution to journalArticlepeer-review

Abstract

We present the results of a study on performance of a reference material for alanine aminotransferase (ALT, EC 2.6.1.2) and the corresponding IFCC-approved method in an interlaboratory trial involving 13 laboratories. The ALT material was partly purified from pig heart (specific activity, 150 kU/g) and was essentially free of six potentially contaminating enzyme activities, including aspartate aminotransferase (EC 2.6.1.1). The partly purified ALT was lyophilized in a triethanolamine-buffered matrix, pH 6.4, containing bovine serum albumin and saccharose. Under these conditions, the predicted yearly loss of activity was 0.02% at 4°C and

Original languageEnglish
Pages (from-to)2365-2371
Number of pages7
JournalClinical Chemistry
Volume38
Issue number12
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Clinical Biochemistry

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