Interleukin 1β and progesterone stimulate activin A expression and secretion from cultured human endometrial stromal cells

Pasquale Florio, Marco Rossi, Paola Viganò, Stefano Luisi, Michela Torricelli, Paulo B. Torres, Anna Maria Di Blasio, Felice Petraglia

Research output: Contribution to journalArticlepeer-review

Abstract

Steroid hormones, cytokines, and growth factors have a major role in evoking local endometrial changes needed for trophoblast implantation. In the present study, the effect of interleukin-1β (IL-1β), 17-β estradiol (E2), and progesterone (Pr) on activin A and follistatin (FS) secretion from cultured human endometrial stromal cells (HESCs) is evaluated. HESCs were obtained from healthy human endometrial samples (n = 8) collected from healthy women. The cells were cultured and stimulated with E2 (10 -7 M, 10-6M), Pr (10-7M, 10-6M), IL-1β (500 pg/mL), IL-1β (500 pg/mL) + E2 (10-6M), and IL-1β (500 pg/mL) + Pr (10-6M). Activin A and FS secretion and mRNA expression were assayed by enzyme-linked immunosorbent assay and semiquantitative reverse transcriptase-polymerase chain reaction, respectively. Pr (10-7 M, 10-6 M) significantly increased activin A secretion and mRNA expression from HESCs, but E2 did not show remarkable effects. The addition of IL-1β (P

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalReproductive Sciences
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Activin A
  • Cytokines
  • Embryo
  • Follistatin
  • Implantation
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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