Interleukin-1β primes interleukin-8-stimulated chemotaxis and elastase release in human neutrophils via its type I receptor

Laura Brandolini, Rita Sergi, Gianfranco Caselli, Diana Boraschi, Massimo Locati, Silvano Sozzani, Riccardo Bertini

Research output: Contribution to journalArticle


Interleukin-1 (IL-1) is a pleiotropic proinflammatory cytokine which binds to human neutrophils (PMN) and can directly or indirectly activate their functions. In this study we show that a brief exposure to IL-1β induces a potentiation of both PMN elastase release and chemotactic response to interleukin-8 (IL-8), the prototype of C-X-C chemokines. Priming by IL-1β was maximal at 100 ng/ml, was completely blocked in the presence of IL-1 receptor antagonist (IL-1ra) and, in the chemotaxis assay, was best observed at suboptimal (3-6 ng/ml) or inactive (0.75 ng/ml) concentrations of IL-8. Priming of PMN by IL-1β was completely blocked by M1, a specific antibody against the type I IL-1 receptor (IL-1RI). On the other hand M22, an antibody directed against the IL-1 decoy type II IL-1 receptor did not affect IL-1β action and slightly increased the priming effect. Thus, exclusively via its type I receptor, IL-1β can act on PMN at multiple levels, by promoting their accumulation in tissues through the induction of chemotactic factors (e.g. IL-8) and the upregulation of adhesion molecules, and by priming their response to chemotactic agonists.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalEuropean Cytokine Network
Issue number2
Publication statusPublished - 1997



  • Chemotaxis
  • Elastase release
  • IL-1 receptors
  • Interleukin-1
  • Interleukin-8
  • Neutrophils
  • Priming

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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