Abstract
Our previous in vitro study demonstrated that interleukin-1 (IL-1) stimulates gene expression and production of leukocyte chemotactic factors, colony-stimulating factors and interleu- kin-6 in human mesangial cells in culture. Here we investigated whether IL-1 regulates its own gene expression in human mesangial cells. Northern blot experiments showed that IL-1 induced IL-1 mRNA expression in a dose-dependent manner. The action of IL-1 on mesangial cells was mediated through the IL-1 receptor type I, which is constitutively expressed by mesangial cells. Recombinant IL-1 receptor antagonist blocked the IL-1 mRNA as well as interleukin-6 and interleukin-8 gene expression induced by IL-1. These data support the crucial role played by IL-1 in regulating mesangial cell cytokine genes within the glomerulus during immunological or inflammatory processes.
| Original language | English |
|---|---|
| Pages (from-to) | 89-92 |
| Number of pages | 4 |
| Journal | Kidney and Blood Pressure Research |
| Volume | 16 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1993 |
Keywords
- Interleukin-1 gene
- Interleukin-1 receptor
- Interleukin-1 receptor antagonist
- Interleukin-6 gene
- Interleukin-8 gene
- Mesangial cells
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Nephrology