Abstract
In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.
| Original language | English |
|---|---|
| Pages (from-to) | 1104-1110 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 152 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 16 1988 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Interleukin-1 induces c-fos protooncogene expression in cultured human endothelial cells. / Colotta, F.; Lampugnani, M. G.; Polentarutti, N.; Dejana, E.; Mantovani, A.
In: Biochemical and Biophysical Research Communications, Vol. 152, No. 3, 16.05.1988, p. 1104-1110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin-1 induces c-fos protooncogene expression in cultured human endothelial cells
AU - Colotta, F.
AU - Lampugnani, M. G.
AU - Polentarutti, N.
AU - Dejana, E.
AU - Mantovani, A.
PY - 1988/5/16
Y1 - 1988/5/16
N2 - In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.
AB - In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.
UR - http://www.scopus.com/inward/record.url?scp=0023951840&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023951840&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)80398-X
DO - 10.1016/S0006-291X(88)80398-X
M3 - Article
C2 - 3259871
AN - SCOPUS:0023951840
VL - 152
SP - 1104
EP - 1110
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -