Interleukin-1 induces c-fos protooncogene expression in cultured human endothelial cells

F. Colotta; M. G. Lampugnani; N. Polentarutti; E. Dejana; A. Mantovani

doi:10.1016/S0006-291X(88)80398-X

Interleukin-1 induces c-fos protooncogene expression in cultured human endothelial cells

F. Colotta, M. G. Lampugnani, N. Polentarutti, E. Dejana, A. Mantovani

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.

Original language	English
Pages (from-to)	1104-1110
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	152
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(88)80398-X
Publication status	Published - May 16 1988

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Interleukin-1 induces c-fos protooncogene expression in cultured human endothelial cells",

abstract = "In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.",

author = "F. Colotta and Lampugnani, {M. G.} and N. Polentarutti and E. Dejana and A. Mantovani",

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AU - Colotta, F.

AU - Lampugnani, M. G.

AU - Polentarutti, N.

AU - Dejana, E.

AU - Mantovani, A.

PY - 1988/5/16

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N2 - In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.

AB - In the present study we have evaluated the expression of c-fos protooncogene in normal human endothelial cells (HEC) by Northern blot analysis. HEC do not show neither constitutive nor cycloheximide-induced expression of c-fos protooncogene. When HEC were treated with cytokines known to modulate a number of specialized functions of these cells, we observed that, unlike interferon-γ, interleukin-1 (IL-1) and tumor necrosis factor (TNF) were able to induce appreciable levels of c-fos in HEC. Both IL-1α and IL-1β induced c-fos transcripts in HEC. Maximal levels of c-fos mRNA induced by IL-1 were found after 1 hour of treatment, with undetectable levels at 4 and 7 hours. c-fos induction in HEC by IL-1 and TNF may play a role in the acquisition of functional properties induced in HEC by these cytokines.

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