Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells

E. Dejana, F. Bertocchi, M. C. Bortolami, A. Regonesi, A. Tonta, F. Breviario, R. Giavazzi

Research output: Contribution to journalArticle

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Abstract

We report that IL 1 acts on the endothelium, inducing a long-lasting increase in its adhesiveness to tumor cells. Selective pretreatment of cultured human umbilical vein endothelial cells (EC) with IL 1 caused a significant increase in adhesion of three human colorectal carcinoma (HT-29, HCC-P2988, and HCC-M1410) cell lines and one human melanoma (A-375) cell line. Tumor necrosis factor (TNF) was as effective as IL 1 in promoting tumor cell adhesion to EC, whereas IFN γ and IL 2 were inactive. The IL 1 and TNF induction of EC adhesiveness was both concentration (threshold concentration 1 U/ml) and time dependent (peak 4-6 h), reversible within 24 h, and blocked by a protein synthesis inhibitor. The IL 1 and TNF action on EC may play a role in tumor cell lodgment.

Original languageEnglish
Pages (from-to)1466-1470
Number of pages5
JournalJournal of Clinical Investigation
Volume82
Issue number4
Publication statusPublished - 1988

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Interleukin-1
Cell Adhesion
Endothelial Cells
Adhesiveness
Tumor Necrosis Factor-alpha
Neoplasms
Cell Line
Protein Synthesis Inhibitors
Human Umbilical Vein Endothelial Cells
Endothelium
Interleukin-2
Colorectal Neoplasms
Melanoma

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Dejana, E., Bertocchi, F., Bortolami, M. C., Regonesi, A., Tonta, A., Breviario, F., & Giavazzi, R. (1988). Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells. Journal of Clinical Investigation, 82(4), 1466-1470.

Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells. / Dejana, E.; Bertocchi, F.; Bortolami, M. C.; Regonesi, A.; Tonta, A.; Breviario, F.; Giavazzi, R.

In: Journal of Clinical Investigation, Vol. 82, No. 4, 1988, p. 1466-1470.

Research output: Contribution to journalArticle

Dejana, E, Bertocchi, F, Bortolami, MC, Regonesi, A, Tonta, A, Breviario, F & Giavazzi, R 1988, 'Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells', Journal of Clinical Investigation, vol. 82, no. 4, pp. 1466-1470.
Dejana E, Bertocchi F, Bortolami MC, Regonesi A, Tonta A, Breviario F et al. Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells. Journal of Clinical Investigation. 1988;82(4):1466-1470.
Dejana, E. ; Bertocchi, F. ; Bortolami, M. C. ; Regonesi, A. ; Tonta, A. ; Breviario, F. ; Giavazzi, R. / Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells. In: Journal of Clinical Investigation. 1988 ; Vol. 82, No. 4. pp. 1466-1470.
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