Interleukin-1 stimulates prostacyclin production by cultured human endothelial cells by increasing arachidonic acid mobilization and conversion

Ferruccio Breviario, Paolo Proserpio, Federico Bertocchi, Maria Grazia Lampugnani, Alberto Mantovani, Elisabetta Dejana

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-1 (IL-1) induced slow, lasting activation of human endothelial cells (EC) to release prostacyclin (PGl2). This was accompanied by endogenous 3H-arachidonic acid (3H-AA) release and by a time-dependent increase in the cells' ability to convert exogenous AA. The continuous presence of IL-1 was not required, but about a 1-hour stimulation with the cytokine was sufficient to trigger the cells to synthesize PGl2 for several hours. The spectrum of 3H-AA conversion shows that, in addition to 6-ketoprostaglandin F, prostaglandin Falso was raised after IL-1. The recovery of PGl2 synthesis after aspirin was faster in IL-1-treated EC than in control cells. These data define some of the characteristics of IL-1 stimulation of PGl2 and suggest that this process is mediated both by endogenous AA mobilization and by an increase in cyclooxygenase activity.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume10
Issue number1
Publication statusPublished - 1990

Keywords

  • Endothelial cells
  • Interleukin-1
  • Prostacyclin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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