TY - JOUR
T1 - Interleukin-1 stimulates prostacyclin production by cultured human endothelial cells by increasing arachidonic acid mobilization and conversion
AU - Breviario, Ferruccio
AU - Proserpio, Paolo
AU - Bertocchi, Federico
AU - Lampugnani, Maria Grazia
AU - Mantovani, Alberto
AU - Dejana, Elisabetta
PY - 1990
Y1 - 1990
N2 - Interleukin-1 (IL-1) induced slow, lasting activation of human endothelial cells (EC) to release prostacyclin (PGl2). This was accompanied by endogenous 3H-arachidonic acid (3H-AA) release and by a time-dependent increase in the cells' ability to convert exogenous AA. The continuous presence of IL-1 was not required, but about a 1-hour stimulation with the cytokine was sufficient to trigger the cells to synthesize PGl2 for several hours. The spectrum of 3H-AA conversion shows that, in addition to 6-ketoprostaglandin F1α, prostaglandin F2αalso was raised after IL-1. The recovery of PGl2 synthesis after aspirin was faster in IL-1-treated EC than in control cells. These data define some of the characteristics of IL-1 stimulation of PGl2 and suggest that this process is mediated both by endogenous AA mobilization and by an increase in cyclooxygenase activity.
AB - Interleukin-1 (IL-1) induced slow, lasting activation of human endothelial cells (EC) to release prostacyclin (PGl2). This was accompanied by endogenous 3H-arachidonic acid (3H-AA) release and by a time-dependent increase in the cells' ability to convert exogenous AA. The continuous presence of IL-1 was not required, but about a 1-hour stimulation with the cytokine was sufficient to trigger the cells to synthesize PGl2 for several hours. The spectrum of 3H-AA conversion shows that, in addition to 6-ketoprostaglandin F1α, prostaglandin F2αalso was raised after IL-1. The recovery of PGl2 synthesis after aspirin was faster in IL-1-treated EC than in control cells. These data define some of the characteristics of IL-1 stimulation of PGl2 and suggest that this process is mediated both by endogenous AA mobilization and by an increase in cyclooxygenase activity.
KW - Endothelial cells
KW - Interleukin-1
KW - Prostacyclin
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M3 - Article
C2 - 2105090
AN - SCOPUS:0025189675
VL - 10
SP - 129
EP - 134
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 1
ER -