Interleukin-10 induces a long-term antigen-specific anergic state in human CD4+ T cells

Hervé Groux, Mike Bigler, Jan E. De Vries, Maria Grazia Roncarolo

Research output: Contribution to journalArticle

Abstract

Human CD4+T cells, activated by allogeneic monocytes in a primary mixed lymphocyte reaction in the presence of exogenous interleukin (IL) 10, specifically failed to proliferative after restimulation with the same alloantigens. A comparable state of T cell unresponsiveness could be induced by activation of CD4+ T cells by cross-linked anti-CD3 monoclonal antibodies (mAbs) in the presence of exogenous IL-10. The anergic T cells failed to produce IL-2, IL-5, IL-10, interferon γ, tumor necrosis factor α, and granulocyte/macrophage colony-stimulating factor. The IL-10-induced anergic state was long-lasting. T cell anergy could not be reversed after restimulation of the cells with anti-CD3 and anti-CD28 mAbs, although CD3 and CD28 expression was normal. In addition, restimulation of anergized T cells with anti-CD3 mAbs induced normal Ca2+ fluxes and resulted in increased CD3, CD28, and class II major histocompatibility complex expression, indicating that calcineurin-mediated signaling occurs in these anergic cells. However, the expression of the IL-2 receptor α claim was not upregulated, which may account for the failure of exogenous IL-2 to reverse the anergic state. Interestingly, anergic T cells and their nonanergic counterparts showed comparable levels of proliferation and cytokine production after activation with phorbol myristate acetate and Ca2+ ionophore; indicating that a direct activation of a protein kinase C-dependent pathway can overcome the tolerizing effect of IL-10. Taken together, these data demonstrate that IL-10 induces T cell anergy and therefore may play an important role in the induction and maintenance of antigen-specific T cell tolerance.

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalJournal of Experimental Medicine
Volume184
Issue number1
DOIs
Publication statusPublished - Jul 1 1996

Fingerprint

Interleukin-10
T-Lymphocytes
Antigens
Monoclonal Antibodies
Interleukin-2
Mixed Lymphocyte Culture Test
Isoantigens
Calcineurin
Interleukin-2 Receptors
Interleukin-5
Ionophores
Tetradecanoylphorbol Acetate
Granulocyte-Macrophage Colony-Stimulating Factor
Major Histocompatibility Complex
Protein Kinase C
Interferons
Monocytes
Tumor Necrosis Factor-alpha
Maintenance
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Interleukin-10 induces a long-term antigen-specific anergic state in human CD4+ T cells. / Groux, Hervé; Bigler, Mike; De Vries, Jan E.; Roncarolo, Maria Grazia.

In: Journal of Experimental Medicine, Vol. 184, No. 1, 01.07.1996, p. 19-29.

Research output: Contribution to journalArticle

Groux, Hervé ; Bigler, Mike ; De Vries, Jan E. ; Roncarolo, Maria Grazia. / Interleukin-10 induces a long-term antigen-specific anergic state in human CD4+ T cells. In: Journal of Experimental Medicine. 1996 ; Vol. 184, No. 1. pp. 19-29.
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