Interleukin-10 suppresses human immunodeficiency virus-1 replication in vitro in cells of the monocyte/macrophage lineage

M. Wayne Saville, Kazayuki Taga, Andrea Foli, Samuel Broder, Giovanna Tosato, Robert Yarchoan

Research output: Contribution to journalArticle

Abstract

The cytokine interleukin-10 (IL-10) has been implicated in the pathogenesis of a number of disease states, including Epstein-Barr virus and human immunodeficiency virus (HIV-1) infections. In the acquired immunodeficiency syndrome (AIDS), it has been suggested that IL-10 may have a deleterious effect by suppressing cell-mediated immunity. However, there are few data on its direct effects on HIV-1 replication. In the present study, we have found that recombinant human IL-10 (rhIL-10), present during days 0 through 2, potently inhibits HIV production in elutriated monocyte/macrophage (M/M) cultures with a 50% inhibitory concentration (IC 50) of approximately 0.03 U/mL. This effect did not appear to be caused by toxicity to M/M because there was no change in cell viability, ability to phagocytose latex beads, or protein synthesis as measured by [ 3H]-leucine incorporation, at doses of rhIL-10 that inhibit viral replication. In addition, lipopolysaccharide- induced production of IL-1β, IL-6, or tumor necrosis factor-α was not affected at these doses, nor were human mononuclear cell proliferative responses to phytohemagglutinin, OKT3 antibody, or tetanus toxoid. HIV-1 replication was similarly decreased by rhIL-10 in the monocytoid line U937 without signs of cellular toxicity. However, these effects required much higher concentrations or rhIL-10, and viral production was only partially suppressed. rhIL-10 also slightly inhibited HIV-induced cytopathicity in ATH- 8, a tetanus toxoid-specific, retrovirally immortalized T-cell line, but had no effect on HIV replication in the H9 and MOLT-4 T cell lines. Thus, rhIL- 10 appears to inhibit HIV replication predominantly in cells of the M/M lineage. This effect may serve to reduce viral production in patients with AIDS. However, additional studies will be needed to more precisely define its physiologic role in this disease.

Original languageEnglish
Pages (from-to)3591-3599
Number of pages9
JournalBlood
Volume83
Issue number12
Publication statusPublished - Jun 15 1994

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Macrophages
Virus Replication
Viruses
Interleukin-10
HIV-1
Monocytes
HIV
Tetanus Toxoid
T-cells
Toxicity
Acquired Immunodeficiency Syndrome
T-Lymphocytes
Muromonab-CD3
Cell Line
Latex
Phytohemagglutinins
In Vitro Techniques
Human Herpesvirus 4
Interleukin-1
Microspheres

ASJC Scopus subject areas

  • Hematology

Cite this

Saville, M. W., Taga, K., Foli, A., Broder, S., Tosato, G., & Yarchoan, R. (1994). Interleukin-10 suppresses human immunodeficiency virus-1 replication in vitro in cells of the monocyte/macrophage lineage. Blood, 83(12), 3591-3599.

Interleukin-10 suppresses human immunodeficiency virus-1 replication in vitro in cells of the monocyte/macrophage lineage. / Saville, M. Wayne; Taga, Kazayuki; Foli, Andrea; Broder, Samuel; Tosato, Giovanna; Yarchoan, Robert.

In: Blood, Vol. 83, No. 12, 15.06.1994, p. 3591-3599.

Research output: Contribution to journalArticle

Saville, MW, Taga, K, Foli, A, Broder, S, Tosato, G & Yarchoan, R 1994, 'Interleukin-10 suppresses human immunodeficiency virus-1 replication in vitro in cells of the monocyte/macrophage lineage', Blood, vol. 83, no. 12, pp. 3591-3599.
Saville, M. Wayne ; Taga, Kazayuki ; Foli, Andrea ; Broder, Samuel ; Tosato, Giovanna ; Yarchoan, Robert. / Interleukin-10 suppresses human immunodeficiency virus-1 replication in vitro in cells of the monocyte/macrophage lineage. In: Blood. 1994 ; Vol. 83, No. 12. pp. 3591-3599.
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