Interleukin-12 is chemotactic for natural killer cells and stimulates their interaction with vascular endothelium

Paola Allavena, Carla Paganin, Dan Zhou, Giancarlo Bianchi, Silvano Sozzani, Alberto Mantovani

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Abstract

We investigated the chemotactic activity of interleukin (IL)-12 on human natural killer (NK) cells and other leukocyte subsets. It was found that IL- 12 induced directional migration of highly enriched preparations of NK cells (>80% CD16+ and CD56+) and CD3-activated T cells (both of CD4 and CD8 subset), but not resting T cells and monocytes. On the contrary, purified polymorphonuclear cells (PMN) showed significant and reproducible chemotactic response to IL-12. The effects of IL-12 on leukocyte migration were observed in a narrow concentration range with a peak at approximately 7.5 ng/mL, and were abrogated by monoclonal antibody (MoAb) anti-IL-12 or after cytokine boiling. We also investigated the interaction of NK cells with vascular endothelium in vitro. Overnight treatment of NK cells with IL-12 augmented their binding to cultured endothelial cells (EC) obtained from umbilical veins. IL-12-increased binding was better observed when resting rather than IL-1-activated EC were used as substratum of adhesion. IL-12-augmented binding of NK cells to resting or IL-1-activated EC involved the LFA-1/ICAM- 1, and VLA-4/VCAM-1 pathways. Thus, by inducing migration and interaction with EC, IL-12 regulates crucial determinants of NK-cell recruitment in tissues.

Original languageEnglish
Pages (from-to)2261-2268
Number of pages8
JournalBlood
Volume84
Issue number7
Publication statusPublished - Oct 1 1994

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Vascular Endothelium
Interleukin-12
Natural Killer Cells
Endothelial cells
Endothelial Cells
T-cells
Interleukin-1
Leukocytes
Integrin alpha4beta1
T-Lymphocytes
Lymphocyte Function-Associated Antigen-1
Umbilical Veins
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Boiling liquids
Monocytes
Cultured Cells
Adhesion
Monoclonal Antibodies
Tissue

ASJC Scopus subject areas

  • Hematology

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Interleukin-12 is chemotactic for natural killer cells and stimulates their interaction with vascular endothelium. / Allavena, Paola; Paganin, Carla; Zhou, Dan; Bianchi, Giancarlo; Sozzani, Silvano; Mantovani, Alberto.

In: Blood, Vol. 84, No. 7, 01.10.1994, p. 2261-2268.

Research output: Contribution to journalArticle

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AU - Paganin, Carla

AU - Zhou, Dan

AU - Bianchi, Giancarlo

AU - Sozzani, Silvano

AU - Mantovani, Alberto

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