Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases

B. Marotti, A. Rocco, P. De Colibus, D. Compare, G. de Nucci, S. Staibano, F. Tatangelo, M. Romano, G. Nardone

Research output: Contribution to journalArticlepeer-review

Abstract

A shift from Th1 (IFN-γ) towards Th2 (IL-4)-type immune response was found in patients with gastric cancer and dysplasia. Recently, IL-13 has been described as a central mediator of Th2-dominant immune response in different inflammatory diseases. Aim and Methods: to analyse, by Enzyme-Linked-Immuno-SPOT (ELISPOT) assay and immunohistochemistry, the IL-13 production of mononuclear cells obtained from gastric biopsies of 19 H. pylori-negative subjects and 23 H. pylori-positive patients. Results: By ELISPOT, we did not find any significant variation of the spot range number of IL-13, IL-4 and IFN-γ secreting cells, irrespective of H. pylori status. After antigenic exposition, the spot range for IL-13, IL-4 and IFN-γ significantly increased (p <.0001) only in H. pylori-positive patients. A prevalent Th1 (IFN-γ) immunoresponse was observed in 2/23 cases with active gastritis, while a prevalent Th2 (IL-13 and IL-4) was detected in 5/23 cases all with atrophic chronic gastritis of whom two with intestinal metaplasia. By immunohistochemistry, IL-13, IL-4 and IFN-γ were detectable in all cases directly related to the inflammatory infiltrate. In the two cases with intestinal metaplasia, IL-13 and IL-4 were localised in both inflammatory and epithelial cells. This immunopattern was confirmed in selected additional 10 cases of H. pylori-positive chronic atrophic gastritis with intestinal metaplasia and 10 cases of intestinal type gastric cancer. Conclusion: These preliminary results suggest that IL-13 could be implicated in the different outcome of H. pylori infection.

Original languageEnglish
Pages (from-to)240-247
Number of pages8
JournalDigestive and Liver Disease
Volume40
Issue number4
DOIs
Publication statusPublished - Apr 2008

Keywords

  • ELISPOT assay
  • Gastric carcinogenesis
  • Helicobacter pylori
  • IL-13
  • Local immune response
  • Th2

ASJC Scopus subject areas

  • Gastroenterology

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