Interleukin-15 favors the expansion of central memory CD8+ T cells in ex vivo generated, antileukemia human cytotoxic T lymphocyte lines

Liane Daudt, Rita MacCario, Franco Locatelli, Ilaria Turin, Lucia Silla, Enrica Montini, Elena Percivalle, Roberto Giugliani, Maria Antonietta Avanzini, Antonia Moretta, Daniela Montagna

Research output: Contribution to journalArticle

Abstract

We demonstrated in previous studies that interleukin (IL) -2 supports in vitro cell proliferation of donor-derived cytotoxic T lymphocyte (CTL) lines directed against different types of leukemia blasts. The aim of this study was to compare the capacity of IL-15 with that of IL-2 in supporting the proliferation and cytotoxic activity of antileukemia CTL cultures, and their influence on T-cell memory compartment differentiation. Antileukemia CTL lines were generated using donor-derived dendritic cells pulsed with apoptotic leukemia blasts, in the presence of IL-12 and IL-7, during the primary culture, and expanded through 2 rounds of leukemia-specific stimulation and 1 round of antigen-independent expansion, each supplemented with either IL-2 or IL-15. Both IL-2-supplemented (IL-2-CTLs) and IL-15-supplemented (IL-15-CTLs) lines contained predominant numbers of CD45RA/CCR7 effector memory (TEM) and CD45RA/CCR7 (TEMRA+) T cells. Significantly higher numbers (P

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalJournal of Immunotherapy
Volume31
Issue number4
DOIs
Publication statusPublished - May 2008

Fingerprint

Interleukin-15
Cytotoxic T-Lymphocytes
Interleukin-2
T-Lymphocytes
Leukemia
Interleukin-7
Interleukin-12
Dendritic Cells
Cell Proliferation
Antigens

Keywords

  • Antileukemia CTLs
  • Antitumor adoptive immunotherapy
  • Common-γ chains cytokines

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Immunology

Cite this

@article{4718a8b64cc449f69159ee33d0e69a8f,
title = "Interleukin-15 favors the expansion of central memory CD8+ T cells in ex vivo generated, antileukemia human cytotoxic T lymphocyte lines",
abstract = "We demonstrated in previous studies that interleukin (IL) -2 supports in vitro cell proliferation of donor-derived cytotoxic T lymphocyte (CTL) lines directed against different types of leukemia blasts. The aim of this study was to compare the capacity of IL-15 with that of IL-2 in supporting the proliferation and cytotoxic activity of antileukemia CTL cultures, and their influence on T-cell memory compartment differentiation. Antileukemia CTL lines were generated using donor-derived dendritic cells pulsed with apoptotic leukemia blasts, in the presence of IL-12 and IL-7, during the primary culture, and expanded through 2 rounds of leukemia-specific stimulation and 1 round of antigen-independent expansion, each supplemented with either IL-2 or IL-15. Both IL-2-supplemented (IL-2-CTLs) and IL-15-supplemented (IL-15-CTLs) lines contained predominant numbers of CD45RA/CCR7 effector memory (TEM) and CD45RA/CCR7 (TEMRA+) T cells. Significantly higher numbers (P",
keywords = "Antileukemia CTLs, Antitumor adoptive immunotherapy, Common-γ chains cytokines",
author = "Liane Daudt and Rita MacCario and Franco Locatelli and Ilaria Turin and Lucia Silla and Enrica Montini and Elena Percivalle and Roberto Giugliani and Avanzini, {Maria Antonietta} and Antonia Moretta and Daniela Montagna",
year = "2008",
month = "5",
doi = "10.1097/CJI.0b013e31816b1092",
language = "English",
volume = "31",
pages = "385--393",
journal = "Journal of Immunotherapy",
issn = "1053-8550",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Interleukin-15 favors the expansion of central memory CD8+ T cells in ex vivo generated, antileukemia human cytotoxic T lymphocyte lines

AU - Daudt, Liane

AU - MacCario, Rita

AU - Locatelli, Franco

AU - Turin, Ilaria

AU - Silla, Lucia

AU - Montini, Enrica

AU - Percivalle, Elena

AU - Giugliani, Roberto

AU - Avanzini, Maria Antonietta

AU - Moretta, Antonia

AU - Montagna, Daniela

PY - 2008/5

Y1 - 2008/5

N2 - We demonstrated in previous studies that interleukin (IL) -2 supports in vitro cell proliferation of donor-derived cytotoxic T lymphocyte (CTL) lines directed against different types of leukemia blasts. The aim of this study was to compare the capacity of IL-15 with that of IL-2 in supporting the proliferation and cytotoxic activity of antileukemia CTL cultures, and their influence on T-cell memory compartment differentiation. Antileukemia CTL lines were generated using donor-derived dendritic cells pulsed with apoptotic leukemia blasts, in the presence of IL-12 and IL-7, during the primary culture, and expanded through 2 rounds of leukemia-specific stimulation and 1 round of antigen-independent expansion, each supplemented with either IL-2 or IL-15. Both IL-2-supplemented (IL-2-CTLs) and IL-15-supplemented (IL-15-CTLs) lines contained predominant numbers of CD45RA/CCR7 effector memory (TEM) and CD45RA/CCR7 (TEMRA+) T cells. Significantly higher numbers (P

AB - We demonstrated in previous studies that interleukin (IL) -2 supports in vitro cell proliferation of donor-derived cytotoxic T lymphocyte (CTL) lines directed against different types of leukemia blasts. The aim of this study was to compare the capacity of IL-15 with that of IL-2 in supporting the proliferation and cytotoxic activity of antileukemia CTL cultures, and their influence on T-cell memory compartment differentiation. Antileukemia CTL lines were generated using donor-derived dendritic cells pulsed with apoptotic leukemia blasts, in the presence of IL-12 and IL-7, during the primary culture, and expanded through 2 rounds of leukemia-specific stimulation and 1 round of antigen-independent expansion, each supplemented with either IL-2 or IL-15. Both IL-2-supplemented (IL-2-CTLs) and IL-15-supplemented (IL-15-CTLs) lines contained predominant numbers of CD45RA/CCR7 effector memory (TEM) and CD45RA/CCR7 (TEMRA+) T cells. Significantly higher numbers (P

KW - Antileukemia CTLs

KW - Antitumor adoptive immunotherapy

KW - Common-γ chains cytokines

UR - http://www.scopus.com/inward/record.url?scp=42649106831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42649106831&partnerID=8YFLogxK

U2 - 10.1097/CJI.0b013e31816b1092

DO - 10.1097/CJI.0b013e31816b1092

M3 - Article

C2 - 18391757

AN - SCOPUS:42649106831

VL - 31

SP - 385

EP - 393

JO - Journal of Immunotherapy

JF - Journal of Immunotherapy

SN - 1053-8550

IS - 4

ER -