Interleukin-15 favors the expansion of central memory CD8+ T cells in ex vivo generated, antileukemia human cytotoxic T lymphocyte lines

Liane Daudt, Rita MacCario, Franco Locatelli, Ilaria Turin, Lucia Silla, Enrica Montini, Elena Percivalle, Roberto Giugliani, Maria Antonietta Avanzini, Antonia Moretta, Daniela Montagna

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrated in previous studies that interleukin (IL) -2 supports in vitro cell proliferation of donor-derived cytotoxic T lymphocyte (CTL) lines directed against different types of leukemia blasts. The aim of this study was to compare the capacity of IL-15 with that of IL-2 in supporting the proliferation and cytotoxic activity of antileukemia CTL cultures, and their influence on T-cell memory compartment differentiation. Antileukemia CTL lines were generated using donor-derived dendritic cells pulsed with apoptotic leukemia blasts, in the presence of IL-12 and IL-7, during the primary culture, and expanded through 2 rounds of leukemia-specific stimulation and 1 round of antigen-independent expansion, each supplemented with either IL-2 or IL-15. Both IL-2-supplemented (IL-2-CTLs) and IL-15-supplemented (IL-15-CTLs) lines contained predominant numbers of CD45RA/CCR7 effector memory (TEM) and CD45RA/CCR7 (TEMRA+) T cells. Significantly higher numbers (P

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalJournal of Immunotherapy
Volume31
Issue number4
DOIs
Publication statusPublished - May 2008

Keywords

  • Antileukemia CTLs
  • Antitumor adoptive immunotherapy
  • Common-γ chains cytokines

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Immunology

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