Interleukin-18 synthesis and secretion by dendritic cells are modulated by interaction with antigen-specific T cells

Stefania Gardella, Cristina Andrei, Sara Costigliolo, Alessandro Poggi, M. Raffaella Zocchi, Anna Rubartelli

Research output: Contribution to journalArticle

Abstract

We show that interleukin-18 is constitutively produced by dendritic cells; synthesis and secretion are poorly affected by maturative stimuli. Challenge of dendritic cells with autologous anti-tetanus toxoid T lymphocytes results in a secretory switch, with induction of secretion of biologically active interleukin-18 and decrease of its intracellular content. Similarly, when dendritic cells are challenged with allospecific T cells a dramatic decrease of intracellular interleukin-18 content occurs, whereas no effects are observed after co-culture with autologous activated T cells. The induction of secretion can be mediated by engagement of CD40 on dendritic cells, as indicated by the increased amount of interleukin-18 in dendritic cell supernatants after CD40 triggering by anti-CD40 antibodies. However, CD40 engagement, unlike from antigen-specific T cells, does not result in reduced intracellular interleukin-18 content, suggesting that this decrease may be mediated by structure(s) involved in antigen recognition.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalJournal of Leukocyte Biology
Volume66
Issue number2
Publication statusPublished - 1999

Keywords

  • Antigen-presenting cells
  • Cytokines
  • Immune response
  • Mixed lymphocyte reaction
  • Tetanus toxoid

ASJC Scopus subject areas

  • Cell Biology

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