Interleukin-1beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer

O. Gallo, A. M. Gori, M. Attanasio, F. Martini, B. Giusti, M. Boddi, E. Gallina, O. Fini, R. Abbate

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Abstract

In patients with advanced head and neck squamous cell carcinoma (HNSC), evidence of cell-mediated immunity and monocyte functional abnormalities has been reported. We studied the production of interleukin 1 beta (IL-lbeta) and interleukin 6 (IL-6) by peripheral blood monocytes from 22 patients with HNSC (12 larynx and ten oral cavity cancers) in comparison with monocyte cytokine production of age-matched healthy subjects. Pure monocytes were incubated with and without lipopolysaccarides (LPS) (10 μg ml-1) for 4 h at 37°C and IL-lbeta and IL-6 concentrations were determined in supernatants by specific ELISA. There was no significant difference in IL-Ibeta levels in monocyte supernatants from cancer in comparison to control subjects; conversely, a higher IL-6 production by unstimulated and LPS-activated cells from HNSC patients than from controls was found. No relationship was observed between cytokine production and cancer stage. The regression analysis evidenced a significant correlation between IL-lbeta and IL-6 monocyte-release in HNSC patients and in controls, so suggesting a possible autocrine control of IL-6 production by other cytokines.

Original languageEnglish
Pages (from-to)465-468
Number of pages4
JournalBritish Journal of Cancer
Volume68
Issue number3
Publication statusPublished - Sep 1993

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Head and Neck Neoplasms
Interleukin-1beta
Monocytes
Interleukin-6
Cytokines
Mouth Neoplasms
Larynx
Cellular Immunity
Mouth
Neoplasms
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Carcinoma, squamous cell of head and neck

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Gallo, O., Gori, A. M., Attanasio, M., Martini, F., Giusti, B., Boddi, M., ... Abbate, R. (1993). Interleukin-1beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. British Journal of Cancer, 68(3), 465-468.

Interleukin-1beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. / Gallo, O.; Gori, A. M.; Attanasio, M.; Martini, F.; Giusti, B.; Boddi, M.; Gallina, E.; Fini, O.; Abbate, R.

In: British Journal of Cancer, Vol. 68, No. 3, 09.1993, p. 465-468.

Research output: Contribution to journalArticle

Gallo, O, Gori, AM, Attanasio, M, Martini, F, Giusti, B, Boddi, M, Gallina, E, Fini, O & Abbate, R 1993, 'Interleukin-1beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer', British Journal of Cancer, vol. 68, no. 3, pp. 465-468.
Gallo O, Gori AM, Attanasio M, Martini F, Giusti B, Boddi M et al. Interleukin-1beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. British Journal of Cancer. 1993 Sep;68(3):465-468.
Gallo, O. ; Gori, A. M. ; Attanasio, M. ; Martini, F. ; Giusti, B. ; Boddi, M. ; Gallina, E. ; Fini, O. ; Abbate, R. / Interleukin-1beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. In: British Journal of Cancer. 1993 ; Vol. 68, No. 3. pp. 465-468.
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