Interleukin-2 bolus therapy induces immediate and selective disappearance from peripheral blood of all lymphocyte subpopulations displaying natural killer activity: Role of cell adhesion to endothelium

Giuseppe Salvo, Paola Samoggia, Rosalba Masciulli, Giovanni Boccoli, Paola Allavena, Gualtiero Mariani, Angela Bullo, Elisabetta Montesoro, Daniela Bulgarini, Paolo Carlini, Enzo Maria Ruggeri, Maria Grazia Arena, Antonio Camagna, Ugo Testa, Federico Calabresi, Cesare Peschle

Research output: Contribution to journalArticle

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Abstract

As early as 10-15 min after the start of a 30 min interleukin-2 (IL-2) infusion, a rapid, virtually complete disappearance of all natural killer (NK) lymphocyte subpopulations (including both CD3- CD56+ and CD3+ CD56+ cells with either alpha/beta or gamma/delta T-cell receptor) was observed from peripheral blood. In contrast, the number of T lymphocytes (CD3+ CD56-) was unmodified for at least 2 h after IL-2 injection. The IL-2-induced, rapid disappearance from peripheral blood of NK and NK-like lymphocytes may be related to their massive adherence to the activated endothelium. In this regard, IL-2 infusion caused a very rapid rise of tumour necrosis factor-alpha (TNF-α) plasma concentration, whereas other cytokines, such as interferon-gamma (IFN-γ), were induced only at later times. In vitro experiments indicated that IL-2, either alone or better combined with TNF-α, exerts a rapid and selective stimulatory effect on NK adhesion to endothelial cells. On the basis of these findings, we suggest that the activation of NK lymphocytes induced by IL-2, alone or combined with TNF-α, plays a key role in mediating the massive and selective adherence of NK and NK-like cells following IL-2 bolus infusion.

Original languageEnglish
Pages (from-to)818-825
Number of pages8
JournalEuropean Journal of Cancer
Volume28
Issue number4-5
DOIs
Publication statusPublished - 1992

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Lymphocyte Subsets
Cell Adhesion
Endothelium
Interleukin-2
Tumor Necrosis Factor-alpha
Therapeutics
Antigen Receptors, T-Cell, gamma-delta
Lymphocytes
Natural Killer Cells
Interferon-gamma
Endothelial Cells
Cytokines
T-Lymphocytes
Injections

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Interleukin-2 bolus therapy induces immediate and selective disappearance from peripheral blood of all lymphocyte subpopulations displaying natural killer activity : Role of cell adhesion to endothelium. / Salvo, Giuseppe; Samoggia, Paola; Masciulli, Rosalba; Boccoli, Giovanni; Allavena, Paola; Mariani, Gualtiero; Bullo, Angela; Montesoro, Elisabetta; Bulgarini, Daniela; Carlini, Paolo; Ruggeri, Enzo Maria; Arena, Maria Grazia; Camagna, Antonio; Testa, Ugo; Calabresi, Federico; Peschle, Cesare.

In: European Journal of Cancer, Vol. 28, No. 4-5, 1992, p. 818-825.

Research output: Contribution to journalArticle

Salvo, G, Samoggia, P, Masciulli, R, Boccoli, G, Allavena, P, Mariani, G, Bullo, A, Montesoro, E, Bulgarini, D, Carlini, P, Ruggeri, EM, Arena, MG, Camagna, A, Testa, U, Calabresi, F & Peschle, C 1992, 'Interleukin-2 bolus therapy induces immediate and selective disappearance from peripheral blood of all lymphocyte subpopulations displaying natural killer activity: Role of cell adhesion to endothelium', European Journal of Cancer, vol. 28, no. 4-5, pp. 818-825. https://doi.org/10.1016/0959-8049(92)90122-I
Salvo, Giuseppe ; Samoggia, Paola ; Masciulli, Rosalba ; Boccoli, Giovanni ; Allavena, Paola ; Mariani, Gualtiero ; Bullo, Angela ; Montesoro, Elisabetta ; Bulgarini, Daniela ; Carlini, Paolo ; Ruggeri, Enzo Maria ; Arena, Maria Grazia ; Camagna, Antonio ; Testa, Ugo ; Calabresi, Federico ; Peschle, Cesare. / Interleukin-2 bolus therapy induces immediate and selective disappearance from peripheral blood of all lymphocyte subpopulations displaying natural killer activity : Role of cell adhesion to endothelium. In: European Journal of Cancer. 1992 ; Vol. 28, No. 4-5. pp. 818-825.
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abstract = "As early as 10-15 min after the start of a 30 min interleukin-2 (IL-2) infusion, a rapid, virtually complete disappearance of all natural killer (NK) lymphocyte subpopulations (including both CD3- CD56+ and CD3+ CD56+ cells with either alpha/beta or gamma/delta T-cell receptor) was observed from peripheral blood. In contrast, the number of T lymphocytes (CD3+ CD56-) was unmodified for at least 2 h after IL-2 injection. The IL-2-induced, rapid disappearance from peripheral blood of NK and NK-like lymphocytes may be related to their massive adherence to the activated endothelium. In this regard, IL-2 infusion caused a very rapid rise of tumour necrosis factor-alpha (TNF-α) plasma concentration, whereas other cytokines, such as interferon-gamma (IFN-γ), were induced only at later times. In vitro experiments indicated that IL-2, either alone or better combined with TNF-α, exerts a rapid and selective stimulatory effect on NK adhesion to endothelial cells. On the basis of these findings, we suggest that the activation of NK lymphocytes induced by IL-2, alone or combined with TNF-α, plays a key role in mediating the massive and selective adherence of NK and NK-like cells following IL-2 bolus infusion.",
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AU - Salvo, Giuseppe

AU - Samoggia, Paola

AU - Masciulli, Rosalba

AU - Boccoli, Giovanni

AU - Allavena, Paola

AU - Mariani, Gualtiero

AU - Bullo, Angela

AU - Montesoro, Elisabetta

AU - Bulgarini, Daniela

AU - Carlini, Paolo

AU - Ruggeri, Enzo Maria

AU - Arena, Maria Grazia

AU - Camagna, Antonio

AU - Testa, Ugo

AU - Calabresi, Federico

AU - Peschle, Cesare

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