Interleukin-2-dependent long-term cultures of low-density lymphocytes allow the proliferation of lymphokine-activated killer cells with natural killer, Tiγ/δ or TNK phenotype

U. Testa, A. Care, E. Montesoro, C. Fossati, G. Giannella, R. Masciulli, M. Fagioli, D. Bulgarini, D. Habetswallner, G. Isacchi, P. G. Pelicci, C. Peschle

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a culture system for "longterm" growth of human lymphokine-activated killer (LAK) cells exhibiting an elevated, wide-spectrum antitumor cytotoxicity. The system allows the exponential growth of monocyte-depleted low-density lymphocytes in the presence of human serum and recombinant human interleukin-2 (103 U/ml), alone or in combination with interleukin-1 α or β (both at 10 U/ml). Eighteen cultures were established from 18 normal adult donors. The membrane phenotypes of the final LAK cell population, assessed by a panel of monoclonal antibodies (mAb), consist of three main types: (a) NKH-1+, Tiα/β-, Tiγ/δ-, and CD3- lymphocytes; (b) NKH-1+, Tiα/β-, Tiγ/δ+, and CD3+ lymphocytes and (c) NKH-1+, Tiα/β+, Tiγ/δ- and CD3+ lymphocytes. Northern blot analysis showed that all these cell populations express relatively high levels of perforin RNA, particularly cells exhibiting the first phenotype. This culture system may provide a tool for cellular and molecular studies on the mechanisms of antitumor cytotoxicity, as well as the basis for new adoptive immunotherapy protocols in advanced cancer.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalCancer Immunology, Immunotherapy
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1990

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

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