We have previously reported that IL-2 is a powerful activator of effector and secretory activities of human monocytes. Monocytes may function as antigen presenting cells. B7-1 plays a critical role as a costimulatory molecule in the process of antigen presentation and T cell response. We investigated the effects of IL-2 on B7-1 mRNA and protein expression in monocytes. Monocytes were isolated by centrifugal ehitriation with a purity of 92 +/- 2%. Very low basal expression of mRNA and protein were detected in medium treated monocytes as determined by Northern blot and flow cytometry analysis respectively. Stimulation of monocytes with IL-2 induced a significant increase of B7-1 relative to the medium control. The induction of B7-1 mRNA by IL-2 was observed as early as 3 h. Dose response experiments showed that 100 U/ml of IL-2 were sufficient to enhance the expression of B7-1 mRNA. Furthermore, we demonstrated that EFN-y synergized with IL-2 in inducing B7-1. These data demonstrate, for the first time, that IL-2 can enhances the expression of B7-1 on human monocytes suggesting that IL-2 by inducing B7-1 may play a role in the ability of monocytes to function as effective antigen presenting cells.
|Publication status||Published - 1996|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology