Interleukin-2 induces the growth of human glioblastoma cells in culture

Enrica Capelli, Monica Civallero, Sergio Barni, Mauro Ceroni, Rosanna Nano

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The ability of the T cell growth factor interleukin-2 (IL-2) to support the proliferation of human glioblastoma cells in short-term cultures was evaluated. A morphological, cytochemical and immunocytochemical analysis was carried out at different times of treatment. In the presence of IL-2 growth of tumor cells was observed. On the contrary, in the absence of IL-2 only few colonies derived from tumor fragments were obtained and these were so after a long time. The immunocytochemical study revealed that IL-2 induces the expression of the IL-2 receptor on human glioblastoma cells. In the presence of IL-2 proliferation of infiltrating lymphoid cells inside the tumor fragments was also observed. Morphological and cytochemical analysis of these cells revealed positivity for acid phosphatase (AP), dihydrofolate reductase (DHFR), dipeptydilaminopeptidase (DAP IV) and negativity for serine esterase. These data are in agreement with our previous study on activated lymphoid subsets. On the other hand, an absence of infiltrating lymphocytes was observed in cultures without IL-2. These results indicate that local treatment of human glioblastoma using IL-2 might produce tumor cell proliferation.

Original languageEnglish
Pages (from-to)3147-3151
Number of pages5
JournalAnticancer Research
Volume19
Issue number4 B
Publication statusPublished - 1999

Fingerprint

Glioblastoma
Interleukin-2
Cell Culture Techniques
Growth
Neoplasms
Lymphocytes
Tetrahydrofolate Dehydrogenase
Interleukin-2 Receptors
Acid Phosphatase
Cell Proliferation

Keywords

  • Cytochemistry
  • Electron microscopy
  • Human glioblastoma cultures
  • IL-2
  • IL-2R
  • Immunocytochemistry

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Capelli, E., Civallero, M., Barni, S., Ceroni, M., & Nano, R. (1999). Interleukin-2 induces the growth of human glioblastoma cells in culture. Anticancer Research, 19(4 B), 3147-3151.

Interleukin-2 induces the growth of human glioblastoma cells in culture. / Capelli, Enrica; Civallero, Monica; Barni, Sergio; Ceroni, Mauro; Nano, Rosanna.

In: Anticancer Research, Vol. 19, No. 4 B, 1999, p. 3147-3151.

Research output: Contribution to journalArticle

Capelli, E, Civallero, M, Barni, S, Ceroni, M & Nano, R 1999, 'Interleukin-2 induces the growth of human glioblastoma cells in culture', Anticancer Research, vol. 19, no. 4 B, pp. 3147-3151.
Capelli E, Civallero M, Barni S, Ceroni M, Nano R. Interleukin-2 induces the growth of human glioblastoma cells in culture. Anticancer Research. 1999;19(4 B):3147-3151.
Capelli, Enrica ; Civallero, Monica ; Barni, Sergio ; Ceroni, Mauro ; Nano, Rosanna. / Interleukin-2 induces the growth of human glioblastoma cells in culture. In: Anticancer Research. 1999 ; Vol. 19, No. 4 B. pp. 3147-3151.
@article{2a5b47367a3f4514837ce92b9890f74f,
title = "Interleukin-2 induces the growth of human glioblastoma cells in culture",
abstract = "The ability of the T cell growth factor interleukin-2 (IL-2) to support the proliferation of human glioblastoma cells in short-term cultures was evaluated. A morphological, cytochemical and immunocytochemical analysis was carried out at different times of treatment. In the presence of IL-2 growth of tumor cells was observed. On the contrary, in the absence of IL-2 only few colonies derived from tumor fragments were obtained and these were so after a long time. The immunocytochemical study revealed that IL-2 induces the expression of the IL-2 receptor on human glioblastoma cells. In the presence of IL-2 proliferation of infiltrating lymphoid cells inside the tumor fragments was also observed. Morphological and cytochemical analysis of these cells revealed positivity for acid phosphatase (AP), dihydrofolate reductase (DHFR), dipeptydilaminopeptidase (DAP IV) and negativity for serine esterase. These data are in agreement with our previous study on activated lymphoid subsets. On the other hand, an absence of infiltrating lymphocytes was observed in cultures without IL-2. These results indicate that local treatment of human glioblastoma using IL-2 might produce tumor cell proliferation.",
keywords = "Cytochemistry, Electron microscopy, Human glioblastoma cultures, IL-2, IL-2R, Immunocytochemistry",
author = "Enrica Capelli and Monica Civallero and Sergio Barni and Mauro Ceroni and Rosanna Nano",
year = "1999",
language = "English",
volume = "19",
pages = "3147--3151",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "4 B",

}

TY - JOUR

T1 - Interleukin-2 induces the growth of human glioblastoma cells in culture

AU - Capelli, Enrica

AU - Civallero, Monica

AU - Barni, Sergio

AU - Ceroni, Mauro

AU - Nano, Rosanna

PY - 1999

Y1 - 1999

N2 - The ability of the T cell growth factor interleukin-2 (IL-2) to support the proliferation of human glioblastoma cells in short-term cultures was evaluated. A morphological, cytochemical and immunocytochemical analysis was carried out at different times of treatment. In the presence of IL-2 growth of tumor cells was observed. On the contrary, in the absence of IL-2 only few colonies derived from tumor fragments were obtained and these were so after a long time. The immunocytochemical study revealed that IL-2 induces the expression of the IL-2 receptor on human glioblastoma cells. In the presence of IL-2 proliferation of infiltrating lymphoid cells inside the tumor fragments was also observed. Morphological and cytochemical analysis of these cells revealed positivity for acid phosphatase (AP), dihydrofolate reductase (DHFR), dipeptydilaminopeptidase (DAP IV) and negativity for serine esterase. These data are in agreement with our previous study on activated lymphoid subsets. On the other hand, an absence of infiltrating lymphocytes was observed in cultures without IL-2. These results indicate that local treatment of human glioblastoma using IL-2 might produce tumor cell proliferation.

AB - The ability of the T cell growth factor interleukin-2 (IL-2) to support the proliferation of human glioblastoma cells in short-term cultures was evaluated. A morphological, cytochemical and immunocytochemical analysis was carried out at different times of treatment. In the presence of IL-2 growth of tumor cells was observed. On the contrary, in the absence of IL-2 only few colonies derived from tumor fragments were obtained and these were so after a long time. The immunocytochemical study revealed that IL-2 induces the expression of the IL-2 receptor on human glioblastoma cells. In the presence of IL-2 proliferation of infiltrating lymphoid cells inside the tumor fragments was also observed. Morphological and cytochemical analysis of these cells revealed positivity for acid phosphatase (AP), dihydrofolate reductase (DHFR), dipeptydilaminopeptidase (DAP IV) and negativity for serine esterase. These data are in agreement with our previous study on activated lymphoid subsets. On the other hand, an absence of infiltrating lymphocytes was observed in cultures without IL-2. These results indicate that local treatment of human glioblastoma using IL-2 might produce tumor cell proliferation.

KW - Cytochemistry

KW - Electron microscopy

KW - Human glioblastoma cultures

KW - IL-2

KW - IL-2R

KW - Immunocytochemistry

UR - http://www.scopus.com/inward/record.url?scp=0033403917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033403917&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 3147

EP - 3151

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 4 B

ER -