Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes

M. G. Roncarolo, M. Zoppo, R. Bacchetta, C. Gabiano, C. Sacchetti, F. Cerutti, P. A. Tovo

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Abstract

In the present study, we investigated the interleukin-2 (IL-2) production and the proliferative responses by peripheral blood mononuclear cells (PBMNC) of 23 children suffering from insulin-dependent diabetes mellitus (IDDM). In addition, the presence of circulating activated T lymphocytes expressing the interleukin-2 receptor (IL-2 R) and HLA-DR antigens was evaluated. The patients were tested at hospital admittance, before starting insulin treatment. Decreased IL-2 production by phytohemagglutinin (PHA)-activated PBMNC of IDDM patients was observed when compared to normal donors. In contrast, the proliferative responses of PBMNC to PHA and Con A were in the normal range. The expression of IL-2 R on patient's lymphocytes was not different from that observed in normal donors, whereas the relative and absolute number of HLA-DR+ T cells was increased. These results confirm the presence in IDDM patients of an imbalanced cellular immune response and demonstrate that the IL-2 deficiency is already present at the diagnosis and is not correlated with insulin administration.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalClinical Immunology and Immunopathology
Volume49
Issue number1
DOIs
Publication statusPublished - 1988

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ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Roncarolo, M. G., Zoppo, M., Bacchetta, R., Gabiano, C., Sacchetti, C., Cerutti, F., & Tovo, P. A. (1988). Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes. Clinical Immunology and Immunopathology, 49(1), 53-62. https://doi.org/10.1016/0090-1229(88)90094-3