Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes

M. G. Roncarolo, M. Zoppo, R. Bacchetta, C. Gabiano, C. Sacchetti, F. Cerutti, P. A. Tovo

Research output: Contribution to journalArticle

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Abstract

In the present study, we investigated the interleukin-2 (IL-2) production and the proliferative responses by peripheral blood mononuclear cells (PBMNC) of 23 children suffering from insulin-dependent diabetes mellitus (IDDM). In addition, the presence of circulating activated T lymphocytes expressing the interleukin-2 receptor (IL-2 R) and HLA-DR antigens was evaluated. The patients were tested at hospital admittance, before starting insulin treatment. Decreased IL-2 production by phytohemagglutinin (PHA)-activated PBMNC of IDDM patients was observed when compared to normal donors. In contrast, the proliferative responses of PBMNC to PHA and Con A were in the normal range. The expression of IL-2 R on patient's lymphocytes was not different from that observed in normal donors, whereas the relative and absolute number of HLA-DR+ T cells was increased. These results confirm the presence in IDDM patients of an imbalanced cellular immune response and demonstrate that the IL-2 deficiency is already present at the diagnosis and is not correlated with insulin administration.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalClinical Immunology and Immunopathology
Volume49
Issue number1
DOIs
Publication statusPublished - 1988

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Interleukin-2 Receptors
Interleukin-2
Type 1 Diabetes Mellitus
Blood Cells
Phytohemagglutinins
HLA-DR Antigens
Tissue Donors
Insulin
T-Lymphocytes
Cellular Immunity
Reference Values
Lymphocytes
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Roncarolo, M. G., Zoppo, M., Bacchetta, R., Gabiano, C., Sacchetti, C., Cerutti, F., & Tovo, P. A. (1988). Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes. Clinical Immunology and Immunopathology, 49(1), 53-62. https://doi.org/10.1016/0090-1229(88)90094-3

Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes. / Roncarolo, M. G.; Zoppo, M.; Bacchetta, R.; Gabiano, C.; Sacchetti, C.; Cerutti, F.; Tovo, P. A.

In: Clinical Immunology and Immunopathology, Vol. 49, No. 1, 1988, p. 53-62.

Research output: Contribution to journalArticle

Roncarolo, MG, Zoppo, M, Bacchetta, R, Gabiano, C, Sacchetti, C, Cerutti, F & Tovo, PA 1988, 'Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes', Clinical Immunology and Immunopathology, vol. 49, no. 1, pp. 53-62. https://doi.org/10.1016/0090-1229(88)90094-3
Roncarolo, M. G. ; Zoppo, M. ; Bacchetta, R. ; Gabiano, C. ; Sacchetti, C. ; Cerutti, F. ; Tovo, P. A. / Interleukin-2 production and interleukin-2 receptor expression in children with newly diagnosed diabetes. In: Clinical Immunology and Immunopathology. 1988 ; Vol. 49, No. 1. pp. 53-62.
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