Abstract
T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.
| Original language | English |
|---|---|
| Pages (from-to) | 383-385 |
| Number of pages | 3 |
| Journal | Nature |
| Volume | 339 |
| Issue number | 6223 |
| Publication status | Published - 1989 |
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Cite this
Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals. / Clerici, Mario; Stocks, Naomi I.; Zajac, Robert A.; Boswell, R. Neal; Bernstein, Denise C.; Mann, Dean L.; Shearer, Gene M.; Berzofsky, Jay A.
In: Nature, Vol. 339, No. 6223, 1989, p. 383-385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals
AU - Clerici, Mario
AU - Stocks, Naomi I.
AU - Zajac, Robert A.
AU - Boswell, R. Neal
AU - Bernstein, Denise C.
AU - Mann, Dean L.
AU - Shearer, Gene M.
AU - Berzofsky, Jay A.
PY - 1989
Y1 - 1989
N2 - T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.
AB - T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.
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M3 - Article
VL - 339
SP - 383
EP - 385
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6223
ER -