Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals

Mario Clerici, Naomi I. Stocks, Robert A. Zajac, R. Neal Boswell, Denise C. Bernstein, Dean L. Mann, Gene M. Shearer, Jay A. Berzofsky

Research output: Contribution to journalArticlepeer-review

Abstract

T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.

Original languageEnglish
Pages (from-to)383-385
Number of pages3
JournalNature
Volume339
Issue number6223
Publication statusPublished - 1989

ASJC Scopus subject areas

  • General

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