Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals

Mario Clerici, Naomi I. Stocks, Robert A. Zajac, R. Neal Boswell, Denise C. Bernstein, Dean L. Mann, Gene M. Shearer, Jay A. Berzofsky

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.

Original languageEnglish
Pages (from-to)383-385
Number of pages3
JournalNature
Volume339
Issue number6223
Publication statusPublished - 1989

Fingerprint

Peptide T
Interleukin-2
HIV
Lymphocytes
Helper-Inducer T-Lymphocytes
Influenza A virus
Peptides
Human Immunodeficiency Virus env Gene Products
Tissue Donors
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • General

Cite this

Clerici, M., Stocks, N. I., Zajac, R. A., Boswell, R. N., Bernstein, D. C., Mann, D. L., ... Berzofsky, J. A. (1989). Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals. Nature, 339(6223), 383-385.

Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals. / Clerici, Mario; Stocks, Naomi I.; Zajac, Robert A.; Boswell, R. Neal; Bernstein, Denise C.; Mann, Dean L.; Shearer, Gene M.; Berzofsky, Jay A.

In: Nature, Vol. 339, No. 6223, 1989, p. 383-385.

Research output: Contribution to journalArticle

Clerici, M, Stocks, NI, Zajac, RA, Boswell, RN, Bernstein, DC, Mann, DL, Shearer, GM & Berzofsky, JA 1989, 'Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals', Nature, vol. 339, no. 6223, pp. 383-385.
Clerici, Mario ; Stocks, Naomi I. ; Zajac, Robert A. ; Boswell, R. Neal ; Bernstein, Denise C. ; Mann, Dean L. ; Shearer, Gene M. ; Berzofsky, Jay A. / Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals. In: Nature. 1989 ; Vol. 339, No. 6223. pp. 383-385.
@article{647be037dbb14b708a54a318ab2457f1,
title = "Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals",
abstract = "T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67{\%} of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90{\%} responded to at least one of the peptides.",
author = "Mario Clerici and Stocks, {Naomi I.} and Zajac, {Robert A.} and Boswell, {R. Neal} and Bernstein, {Denise C.} and Mann, {Dean L.} and Shearer, {Gene M.} and Berzofsky, {Jay A.}",
year = "1989",
language = "English",
volume = "339",
pages = "383--385",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6223",

}

TY - JOUR

T1 - Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals

AU - Clerici, Mario

AU - Stocks, Naomi I.

AU - Zajac, Robert A.

AU - Boswell, R. Neal

AU - Bernstein, Denise C.

AU - Mann, Dean L.

AU - Shearer, Gene M.

AU - Berzofsky, Jay A.

PY - 1989

Y1 - 1989

N2 - T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.

AB - T LYMPHOCYTES from mice1 and healthy humans2 immunized against the human immunodeficiency virus (HIV) envelope have recently been shown to recognize two antigenic regions of the gp160 HIV-envelope protein which have been located on the basis of amphipathicity3-6. In HIV-infected humans, T-cell proliferative responses are lost soon after infection7,8. Here we demonstrate that interleukin-2 production is often retained even when proliferative activity is absent, and that it can be used to monitor T-helper cell responses by HIV-seropositive donors. We use this approach to investigate the T-helper cell response of 42 asymptomatic HIV-seropositive patients to four synthetic gp160 peptides and to influenza A virus, an antigen requiring intact CD4 T-helper cell function. As many as 67% of the HIV-seropositive donors who retain responsiveness to influenza A virus respond to a single peptide, and 85-90% responded to at least one of the peptides.

UR - http://www.scopus.com/inward/record.url?scp=0024385934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024385934&partnerID=8YFLogxK

M3 - Article

VL - 339

SP - 383

EP - 385

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6223

ER -