Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells

Daniela De Totero, Raffaella Meazza, Simona Zupo, Giovanna Cutrona, Serena Matis, Monica Colombo, Enrico Balleari, Ivana Pierri, Marina Fabbi, Matteo Capaia, Bruno Azzarone, Marco Gobbi, Manlio Ferrarini, Silvano Ferrini

Research output: Contribution to journalArticle

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Abstract

Interleukin-21 (IL-21) is a member of the IL-2 cytokine family, which mediates proliferation or growth arrest and apoptosis of normal B cells, depending on their activation state. Here we demonstrate that surface IL-21 receptor (R) is expressed at variable levels by chronic lymphocytic leukemia (CLL) B cells freshly isolated from 33 different patients. IL-21R expression was up-regulated following cell stimulation via surface CD40. Therefore, IL-21 effects were more evident in CD40-activated CLL B cells. IL-21 induced an early signaling cascade in CLL B cells, which included JAK-1 and JAK-3 autophosphorylation and tyrosine phosphorylation of STAT-1, STAT-3, and STAT-5. IL-21 signaling failed to stimulate CLL B-cell proliferation, but induced their apoptosis. In addition, IL-21 counteracted the proliferative and antiapoptotic signals delivered by IL-15 to CLL B cells. IL-21-mediated apoptosis involved activation of caspase-8 and caspase-3, cleavage of Bid to its active form t-Bid, and cleavage of PARP and of p27Kip-1. Recent data indicate that CLL B cells require interaction with the microenvironment for their survival and expansion. The present findings thus provide a set of new mechanisms involved in the balance between cell-survival and apoptotic signals in CLL B cells.

Original languageEnglish
Pages (from-to)3708-3715
Number of pages8
JournalBlood
Volume107
Issue number9
DOIs
Publication statusPublished - May 1 2006

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Interleukin-21 Receptors
B-Cell Chronic Lymphocytic Leukemia
Cells
Apoptosis
Chemical activation
Interleukin-15
Phosphorylation
Caspase 8
Cell proliferation
Cell Communication
Caspase 3
Interleukin-2
Tyrosine
interleukin-21
Cell Survival
B-Lymphocytes
Cell Proliferation
Cytokines

ASJC Scopus subject areas

  • Hematology

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Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells. / De Totero, Daniela; Meazza, Raffaella; Zupo, Simona; Cutrona, Giovanna; Matis, Serena; Colombo, Monica; Balleari, Enrico; Pierri, Ivana; Fabbi, Marina; Capaia, Matteo; Azzarone, Bruno; Gobbi, Marco; Ferrarini, Manlio; Ferrini, Silvano.

In: Blood, Vol. 107, No. 9, 01.05.2006, p. 3708-3715.

Research output: Contribution to journalArticle

De Totero, Daniela ; Meazza, Raffaella ; Zupo, Simona ; Cutrona, Giovanna ; Matis, Serena ; Colombo, Monica ; Balleari, Enrico ; Pierri, Ivana ; Fabbi, Marina ; Capaia, Matteo ; Azzarone, Bruno ; Gobbi, Marco ; Ferrarini, Manlio ; Ferrini, Silvano. / Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells. In: Blood. 2006 ; Vol. 107, No. 9. pp. 3708-3715.
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