Interleukin-6 expression by osteoblast-like MG63 cells challenged with four acrylic bone cements

E. Cenni, D. Granchi, G. Ciapetti, S. Stea, L. Savarino, A. Corradini

Research output: Contribution to journalArticlepeer-review


Periprosthetic osteolysis is a major clinical problem in total hip and total knee arthroplasty and polymethylmethacrylate (PMMA) is a possible etiologic factor. Recently, increasing importance was ascribed to interleukin-6 (IL-6) as an agent favouring bone resorption. The aim of the present study was to investigate the role of bone cements on IL-6 production by MG63. The effect of four acrylic bone cements (Sulfix-60®, CMW 1®, CMW 2®, and CMW 3®) on the protein release and mRNA expression of IL-6 in osteoblast-like cell line MG63 was examined using IL-1 β (0.2 μg ml-1) as the positive control. The extracts in minimum essential medium (MEM) of the cements were tested, following 1-h and 7-day curing. CMW 1® and CMW 2® significantly increased the IL-6 release into the culture media (p <0.01). The cells incubated with Sulfix-60® and CMW 3® produced no significantly different levels of IL-6 than the basal production. A positive correlation was found between the concentration of IL-6 and the contents of benzoylperoxide (p = 0.0003) and barium sulphate (p <0.0001). MG63 expressed IL-6 mRNA constitutively, as demonstrated by the positivity of the negative controls too. We conclude that CMW 1® and CMW 2® increase the production of IL-6 in MG63 cells. The response to Sulfix-60® and CMW 3® is not significantly greater than the negative control.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalJournal of Biomaterials Science, Polymer Edition
Issue number2
Publication statusPublished - 2001


  • Bone cement
  • Interleukin-6
  • MG63
  • Osteoblast

ASJC Scopus subject areas

  • Biophysics


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