Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line

Priscilla Biswas, Fanny Delfanti, Sergio Bernasconi, Manuela Mengozzi, Manuela Cota, Nadia Polentarutti, Alberto Mantovani, Adriano Lazzarin, Silvano Sozzani, Guido Poli

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Induction of chemokine gene expression from peripheral blood mononuclear cells (PBMCs) stimulated by proinflammatory cytokines plays an important role in both wound repair and response to infectious agents. In the present study, we show that the proinflammatory cytokine interleukin-6 (IL-6) potently induced mRNA expression and secretion of the CC chemokine monocyte chemotactic protein 1 (MCP-1) in PBMCs. In addition, because human immunodeficiency virus (HIV) infection in vivo and in vitro has been shown to dysregulate the production of and/or the response to cytokines, PBMCs from both healthy uninfected and HIV-infected individuals were studied for their constitutive and IL-6-induced expression of MCP-1. No substantial differences were observed between the two groups of individuals. In addition, IL-6 upregulated MCP-1 expression in the promonocytic cell line U937 and in its chronically HIV-infected counterpart, U1. In these cell lines, IL-6 selectively induced MCP-1 and not other chemokines, including regulated upon activation normal T cells expressed and secreted (RANTES), macrophage inflammatory protein-1α (MIP-1α), MIP-1β, and IL-8. IL-6 induction of MCP- 1 was partially inhibited by hydrocortisone in U1 cells. Thus, IL-6 activates PBMCs to secrete MCP-1, a CC chemokine pivotal for monocyte recruitment in tissue and organs in which important inflammatory events occur.

Original languageEnglish
Pages (from-to)258-265
Number of pages8
JournalBlood
Volume91
Issue number1
Publication statusPublished - Jan 1 1998

Fingerprint

U937 Cells
Chemokine CCL2
Interleukin-6
Blood Cells
Blood
Cells
Cell Line
Viruses
Macrophage Inflammatory Proteins
CC Chemokines
HIV
Cytokines
Chemokines
T-cells
Virus Diseases
Interleukin-8
Gene expression
Hydrocortisone
Monocytes
Repair

ASJC Scopus subject areas

  • Hematology

Cite this

Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line. / Biswas, Priscilla; Delfanti, Fanny; Bernasconi, Sergio; Mengozzi, Manuela; Cota, Manuela; Polentarutti, Nadia; Mantovani, Alberto; Lazzarin, Adriano; Sozzani, Silvano; Poli, Guido.

In: Blood, Vol. 91, No. 1, 01.01.1998, p. 258-265.

Research output: Contribution to journalArticle

Biswas, Priscilla ; Delfanti, Fanny ; Bernasconi, Sergio ; Mengozzi, Manuela ; Cota, Manuela ; Polentarutti, Nadia ; Mantovani, Alberto ; Lazzarin, Adriano ; Sozzani, Silvano ; Poli, Guido. / Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line. In: Blood. 1998 ; Vol. 91, No. 1. pp. 258-265.
@article{55ecbc88b413430ba5b0d3ea2929174c,
title = "Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line",
abstract = "Induction of chemokine gene expression from peripheral blood mononuclear cells (PBMCs) stimulated by proinflammatory cytokines plays an important role in both wound repair and response to infectious agents. In the present study, we show that the proinflammatory cytokine interleukin-6 (IL-6) potently induced mRNA expression and secretion of the CC chemokine monocyte chemotactic protein 1 (MCP-1) in PBMCs. In addition, because human immunodeficiency virus (HIV) infection in vivo and in vitro has been shown to dysregulate the production of and/or the response to cytokines, PBMCs from both healthy uninfected and HIV-infected individuals were studied for their constitutive and IL-6-induced expression of MCP-1. No substantial differences were observed between the two groups of individuals. In addition, IL-6 upregulated MCP-1 expression in the promonocytic cell line U937 and in its chronically HIV-infected counterpart, U1. In these cell lines, IL-6 selectively induced MCP-1 and not other chemokines, including regulated upon activation normal T cells expressed and secreted (RANTES), macrophage inflammatory protein-1α (MIP-1α), MIP-1β, and IL-8. IL-6 induction of MCP- 1 was partially inhibited by hydrocortisone in U1 cells. Thus, IL-6 activates PBMCs to secrete MCP-1, a CC chemokine pivotal for monocyte recruitment in tissue and organs in which important inflammatory events occur.",
author = "Priscilla Biswas and Fanny Delfanti and Sergio Bernasconi and Manuela Mengozzi and Manuela Cota and Nadia Polentarutti and Alberto Mantovani and Adriano Lazzarin and Silvano Sozzani and Guido Poli",
year = "1998",
month = "1",
day = "1",
language = "English",
volume = "91",
pages = "258--265",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Interleukin-6 induces monocyte chemotactic protein-1 in peripheral blood mononuclear cells and in the U937 cell line

AU - Biswas, Priscilla

AU - Delfanti, Fanny

AU - Bernasconi, Sergio

AU - Mengozzi, Manuela

AU - Cota, Manuela

AU - Polentarutti, Nadia

AU - Mantovani, Alberto

AU - Lazzarin, Adriano

AU - Sozzani, Silvano

AU - Poli, Guido

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Induction of chemokine gene expression from peripheral blood mononuclear cells (PBMCs) stimulated by proinflammatory cytokines plays an important role in both wound repair and response to infectious agents. In the present study, we show that the proinflammatory cytokine interleukin-6 (IL-6) potently induced mRNA expression and secretion of the CC chemokine monocyte chemotactic protein 1 (MCP-1) in PBMCs. In addition, because human immunodeficiency virus (HIV) infection in vivo and in vitro has been shown to dysregulate the production of and/or the response to cytokines, PBMCs from both healthy uninfected and HIV-infected individuals were studied for their constitutive and IL-6-induced expression of MCP-1. No substantial differences were observed between the two groups of individuals. In addition, IL-6 upregulated MCP-1 expression in the promonocytic cell line U937 and in its chronically HIV-infected counterpart, U1. In these cell lines, IL-6 selectively induced MCP-1 and not other chemokines, including regulated upon activation normal T cells expressed and secreted (RANTES), macrophage inflammatory protein-1α (MIP-1α), MIP-1β, and IL-8. IL-6 induction of MCP- 1 was partially inhibited by hydrocortisone in U1 cells. Thus, IL-6 activates PBMCs to secrete MCP-1, a CC chemokine pivotal for monocyte recruitment in tissue and organs in which important inflammatory events occur.

AB - Induction of chemokine gene expression from peripheral blood mononuclear cells (PBMCs) stimulated by proinflammatory cytokines plays an important role in both wound repair and response to infectious agents. In the present study, we show that the proinflammatory cytokine interleukin-6 (IL-6) potently induced mRNA expression and secretion of the CC chemokine monocyte chemotactic protein 1 (MCP-1) in PBMCs. In addition, because human immunodeficiency virus (HIV) infection in vivo and in vitro has been shown to dysregulate the production of and/or the response to cytokines, PBMCs from both healthy uninfected and HIV-infected individuals were studied for their constitutive and IL-6-induced expression of MCP-1. No substantial differences were observed between the two groups of individuals. In addition, IL-6 upregulated MCP-1 expression in the promonocytic cell line U937 and in its chronically HIV-infected counterpart, U1. In these cell lines, IL-6 selectively induced MCP-1 and not other chemokines, including regulated upon activation normal T cells expressed and secreted (RANTES), macrophage inflammatory protein-1α (MIP-1α), MIP-1β, and IL-8. IL-6 induction of MCP- 1 was partially inhibited by hydrocortisone in U1 cells. Thus, IL-6 activates PBMCs to secrete MCP-1, a CC chemokine pivotal for monocyte recruitment in tissue and organs in which important inflammatory events occur.

UR - http://www.scopus.com/inward/record.url?scp=0031975143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031975143&partnerID=8YFLogxK

M3 - Article

C2 - 9414293

AN - SCOPUS:0031975143

VL - 91

SP - 258

EP - 265

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -