Interleukin-6 production of uraemic haemodialysed patients: Effects of different membranes

B. Memoli, C. Libetta, T. Rampino, W. De Simone, S. Meccariello, P. Stangerlin, A. Dal Canton, V. E. Andreucci

Research output: Contribution to journalArticle

Abstract

Interleukin-6 (Il-6) has a complex spectrum of biological activities (growth and differentiation of B cells and synthesis of acute-phase proteins by liver). To evaluate the role of this cytokine in the inflammatory response induced by blood interaction with haemodialysis membranes, we have investigated Il-6 synthesis and release in supernatant of 24-h cultured peripheral blood mononuclear cells (PBMC) isolated from ten haemodialysed patients and eight healthy control subjects. In haemodialysed patients, blood samples were drawn before and after their usual dialytic treatment with euprophane membranes and following 1 and 2 months with polymethylmethacrylate (PMMA) membranes. Il-6 was determined by 72-h incubation of serial dilutions of PBMC supernatant with Il-6-dependent cell line 7TD1; dilutions of rIl-6 were included as standard. Compared to Il-6 synthesis in control subjects (3.3 ± 2.8 U/ml) the patients usually haemodialysed with euprophane membranes showed significantly greater values (9.8 ± 4.5 U/ml, P

Original languageEnglish
Pages (from-to)96-98
Number of pages3
JournalNephrology Dialysis Transplantation
Volume6
Issue numberSUPPL. 2
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

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    Memoli, B., Libetta, C., Rampino, T., De Simone, W., Meccariello, S., Stangerlin, P., Dal Canton, A., & Andreucci, V. E. (1991). Interleukin-6 production of uraemic haemodialysed patients: Effects of different membranes. Nephrology Dialysis Transplantation, 6(SUPPL. 2), 96-98.