Interleukin-8 and CXCR1 Receptor Functional Polymorphisms and Susceptibility to Acute Pyelonephritis

Lina Artifoni, Susanna Negrisolo, Giovanni Montini, Pietro Zucchetta, Pier Paolo Molinari, Walburga Cassar, Roberta Destro, Franca Anglani, Waifro Rigamonti, Graziella Zacchello, Luisa Murer

Research output: Contribution to journalArticle

Abstract

Purpose: We performed a case-control study in children diagnosed by the first episode of upper urinary tract infection with or without vesicoureteral reflux to evaluate the association of functional polymorphism of interleukin-8 (-251A>T and +2767A>G), and its receptor CXCR1 (+2607G>C). Materials and Methods: Genomic DNA was obtained from 265 children with a clinical and laboratory diagnosis of urinary tract infection who were recruited in northeast Italy. The children were subdivided as 173 who were dimercapto-succinic acid scan positive with positive static renal scintigraphy in acute conditions, consistent with the diagnosis of acute pyelonephritis, and 92 who were dimercapto-succinic acid scan negative. Genetic analysis for the same polymorphisms was also extended to a control population of 106 umbilical cord DNA samples. Results: Statistical analysis of genotype data showed that 1) the tested populations were in Hardy-Weinberg equilibrium, 2) there were significant differences between the dimercapto-succinic acid scan positive and negative groups (p = 0.049), and the dimercapto-succinic acid scan positive group vs controls (p = 0.032) in terms of interleukin-8 -251A>T polymorphism frequency, 3) there was also a significant difference in the distribution of IL-8 -251A>T and +2767A>G polymorphisms between dimercapto-succinic acid scan positive and negative children in the subgroup without vesicoureteral reflux (p = 0.03 and 0.02, respectively) and 4) no significant differences were found in the frequency of the distribution of CXCR1 +2607G>C polymorphism in all groups. Conclusions: These data suggest that the gene for the proinflammatory chemokine interleukin-8 is involved in susceptibility to acute pyelonephritis during upper urinary tract infection in children with or without vesicoureteral reflux.

Original languageEnglish
Pages (from-to)1102-1106
Number of pages5
JournalJournal of Urology
Volume177
Issue number3
DOIs
Publication statusPublished - Mar 2007

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Interleukin-8A Receptors
Interleukin-8 Receptors
Pyelonephritis
Succinic Acid
Interleukin-8
Vesico-Ureteral Reflux
Urinary Tract Infections
Statistical Data Interpretation
Clinical Laboratory Techniques
Umbilical Cord
DNA
Chemokines
Radionuclide Imaging
Italy
Population
Case-Control Studies
Genotype
Kidney
Control Groups
Genes

Keywords

  • chemokines
  • genetic
  • polymorphism
  • pyelonephritis
  • urinary tract
  • urinary tract infections

ASJC Scopus subject areas

  • Urology

Cite this

Artifoni, L., Negrisolo, S., Montini, G., Zucchetta, P., Molinari, P. P., Cassar, W., ... Murer, L. (2007). Interleukin-8 and CXCR1 Receptor Functional Polymorphisms and Susceptibility to Acute Pyelonephritis. Journal of Urology, 177(3), 1102-1106. https://doi.org/10.1016/j.juro.2006.10.037

Interleukin-8 and CXCR1 Receptor Functional Polymorphisms and Susceptibility to Acute Pyelonephritis. / Artifoni, Lina; Negrisolo, Susanna; Montini, Giovanni; Zucchetta, Pietro; Molinari, Pier Paolo; Cassar, Walburga; Destro, Roberta; Anglani, Franca; Rigamonti, Waifro; Zacchello, Graziella; Murer, Luisa.

In: Journal of Urology, Vol. 177, No. 3, 03.2007, p. 1102-1106.

Research output: Contribution to journalArticle

Artifoni, L, Negrisolo, S, Montini, G, Zucchetta, P, Molinari, PP, Cassar, W, Destro, R, Anglani, F, Rigamonti, W, Zacchello, G & Murer, L 2007, 'Interleukin-8 and CXCR1 Receptor Functional Polymorphisms and Susceptibility to Acute Pyelonephritis', Journal of Urology, vol. 177, no. 3, pp. 1102-1106. https://doi.org/10.1016/j.juro.2006.10.037
Artifoni, Lina ; Negrisolo, Susanna ; Montini, Giovanni ; Zucchetta, Pietro ; Molinari, Pier Paolo ; Cassar, Walburga ; Destro, Roberta ; Anglani, Franca ; Rigamonti, Waifro ; Zacchello, Graziella ; Murer, Luisa. / Interleukin-8 and CXCR1 Receptor Functional Polymorphisms and Susceptibility to Acute Pyelonephritis. In: Journal of Urology. 2007 ; Vol. 177, No. 3. pp. 1102-1106.
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AU - Montini, Giovanni

AU - Zucchetta, Pietro

AU - Molinari, Pier Paolo

AU - Cassar, Walburga

AU - Destro, Roberta

AU - Anglani, Franca

AU - Rigamonti, Waifro

AU - Zacchello, Graziella

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