Intermediate and low abundant protein analysis of Vitamin D deficient obese and non-obese subjects by MALDI-profiling

Nasser M. Al-Daghri, Enrica Torretta, Daniele Capitanio, Chiara Fania, Franca Rosa Guerini, Shaun B. Sabico, Mario Clerici, Cecilia Gelfi

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Abstract

Obesity is a pathological condition caused by genetic and environmental factors, including vitamin D deficiency, which increases the risk of developing cardiovascular disorders and diabetes. This case-control study was designed to verify whether serum profiles could be identified differentiating obese and non-obese Saudis characterized by vitamin D deficiency and pathological levels of triglycerides, high-density lipoprotein cholesterol and high total cholesterol levels. The serum protein profiles of 64 vitamin D deficient (serum 25(OH)D < 50nmol/L) individuals with metabolic syndrome and with (n = 31; BMI ≥ 30) or without (n = 33; BMI < 30) obesity were analyzed by a quantitative label-free mass spectrometry approach (MALDI-profiling), combined with different serum immunodepletion strategies (Human7 and Human14 immuno-chromatographies), to analyze the intermediate- and low-abundant protein components. The analysis of intermediate-abundant proteins (Human7) in obese vs. non-obese subjects identified 14 changed peaks (p < 0.05) in the m/z range 1500-35000. Furthermore, the Human14 depletion provided new profiles related to obesity (121 changed peaks). Among changed peaks, 11 were identified in the m/z range 1500-4000 Da by high-resolution tandem mass spectrometry, belonging to apolipoprotein CIII, apolipoprotein B100, alpha-1-antichymotrypsin and complement C3. Data herein show that distinct protein profiles identify specific peptides belonging to lipid metabolism and inflammation processes that are associated with obesity and vitamin D deficiency.

Original languageEnglish
Article number12633
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Vitamin D
Vitamin D Deficiency
Obesity
Proteins
alpha 1-Antichymotrypsin
Serum
Apolipoprotein C-III
Complement C3
Apolipoproteins
Tandem Mass Spectrometry
Hypercholesterolemia
Lipid Metabolism
HDL Cholesterol
Case-Control Studies
Chromatography
Blood Proteins
Mass Spectrometry
Triglycerides
Inflammation

ASJC Scopus subject areas

  • General

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Intermediate and low abundant protein analysis of Vitamin D deficient obese and non-obese subjects by MALDI-profiling. / Al-Daghri, Nasser M.; Torretta, Enrica; Capitanio, Daniele; Fania, Chiara; Guerini, Franca Rosa; Sabico, Shaun B.; Clerici, Mario; Gelfi, Cecilia.

In: Scientific Reports, Vol. 7, No. 1, 12633, 01.12.2017.

Research output: Contribution to journalArticle

Al-Daghri, Nasser M. ; Torretta, Enrica ; Capitanio, Daniele ; Fania, Chiara ; Guerini, Franca Rosa ; Sabico, Shaun B. ; Clerici, Mario ; Gelfi, Cecilia. / Intermediate and low abundant protein analysis of Vitamin D deficient obese and non-obese subjects by MALDI-profiling. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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