Intermittent exposure to ethanol vapor affects osteoblast behaviour more severely than estrogen deficiency does. In vitro study on rat osteoblasts

Paola Torricelli, Milena Fini, Gianluca Giavaresi, Veronica Borsari, Lia Rimondini, Roberto Rimondini, Antonio Carrassi, Roberto Giardino

Research output: Contribution to journalArticlepeer-review

Abstract

With rising rates of alcohol consumption acute and chronic damage from alcohol is expected to increase all over the world. Habitual excessive alcohol consumption is associated with pathological effects on bone. The aim of the present in vitro study was to investigate comparatively the proliferation and synthetic activity of osteoblasts (OB) isolated from the trabecular bone of rats previously exposed to 7-week intermittent exposure to ethanol vapor, sham-aged rats and long-term estrogen deficient rats. Cell proliferation (WST1) and synthesis of alkaline phosphatase (ALP), osteocalcin (OC), collagen I (CICP), transforming growth factor beta1 (TGF-β1), interleukin-6 (IL-6), tumor necrosis factor alfa (TNFα) were measured at 3, 7 and 14 days of culture. Osteoblast proliferation rate and TGF-β1, IL-6 and TNFα syntheses were significantly affected by alcohol exposure. Estrogen deficiency and alcohol consumption share many common pathophysiological mechanisms of damage to bone, but alcohol affects OB proliferation and TNFα synthesis significantly more than menopause does. Therefore, these in vitro data suggest that alcohol has even more deleterious effects on bone than estrogen deficiency does.

Original languageEnglish
Pages (from-to)168-176
Number of pages9
JournalToxicology
Volume237
Issue number1-3
DOIs
Publication statusPublished - Jul 31 2007

Keywords

  • Alcohol
  • Estrogen-deficiency
  • Osteoblast
  • Osteoporosis
  • Rats

ASJC Scopus subject areas

  • Toxicology

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