Intermittent high glucose enhances apoptosis in human umbilical vein endothelial cells in culture

A. Risso, F. Mercuri, L. Quagliaro, G. Damante, A. Ceriello

Research output: Contribution to journalArticle

296 Citations (Scopus)

Abstract

To explore the effect of fluctuating glucose on endothelial cells, human umbilical vein endothelial cells were incubated for 14 days in media containing different glucose concentrations: 5 mmol/l, 20 mmol/l, or a daily alternating 5 or 20 mmol/l glucose. Apoptosis was studied by different methods: viability assay, cell cycle analysis, DNA fragmentation, and morphological analysis. Furthermore, the levels of Bcl-2 and Bax, well known proteins involved in apoptosis, were evaluated. Stable high glucose induced apoptosis in human umbilical vein endothelial cells, a phenomenon accompanied by a significant decrease of Bcl-2 and a simultaneous increase of Bax expression. However, apoptosis was enhanced in human umbilical vein endothelial cells exposed to intermittent, rather than constant, high glucose concentration. In this condition, Bcl-2 was not detectable, whereas Bax expression was significantly enhanced. These findings suggest that variability in glycemic control could be more deleterious to endothelial cells than a constant high concentration of glucose.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume281
Issue number5 44-5
Publication statusPublished - 2001

Fingerprint

Endothelial cells
Human Umbilical Vein Endothelial Cells
Cell culture
Cell Culture Techniques
Apoptosis
Glucose
Endothelial Cells
DNA Fragmentation
Assays
Cell Cycle
Cells
DNA
Proteins

Keywords

  • Apoptosis
  • Bax protein
  • Bcl-2 protein
  • Endothelium
  • Intermittent glucose

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Physiology (medical)

Cite this

Intermittent high glucose enhances apoptosis in human umbilical vein endothelial cells in culture. / Risso, A.; Mercuri, F.; Quagliaro, L.; Damante, G.; Ceriello, A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 281, No. 5 44-5, 2001.

Research output: Contribution to journalArticle

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