Intermittent high glucose enhances apoptosis in human umbilical vein endothelial cells in culture

A. Risso, F. Mercuri, L. Quagliaro, G. Damante, A. Ceriello

Research output: Contribution to journalArticle

Abstract

To explore the effect of fluctuating glucose on endothelial cells, human umbilical vein endothelial cells were incubated for 14 days in media containing different glucose concentrations: 5 mmol/l, 20 mmol/l, or a daily alternating 5 or 20 mmol/l glucose. Apoptosis was studied by different methods: viability assay, cell cycle analysis, DNA fragmentation, and morphological analysis. Furthermore, the levels of Bcl-2 and Bax, well known proteins involved in apoptosis, were evaluated. Stable high glucose induced apoptosis in human umbilical vein endothelial cells, a phenomenon accompanied by a significant decrease of Bcl-2 and a simultaneous increase of Bax expression. However, apoptosis was enhanced in human umbilical vein endothelial cells exposed to intermittent, rather than constant, high glucose concentration. In this condition, Bcl-2 was not detectable, whereas Bax expression was significantly enhanced. These findings suggest that variability in glycemic control could be more deleterious to endothelial cells than a constant high concentration of glucose.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume281
Issue number5 44-5
Publication statusPublished - 2001

Keywords

  • Apoptosis
  • Bax protein
  • Bcl-2 protein
  • Endothelium
  • Intermittent glucose

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Physiology (medical)

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