Internal deletions of amino acids 29-42 of human interleukin-6 (IL-6) differentially affect bioactivity and folding

Rosaria Arcone, Veronique Fontaine, Iolanda Coto, Jean Content, Gennaro Ciliberto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Internal deletions of the human interleukin-6 (IL-6) cDNA have been generated in the region encoding residues 29 to 42. Mutant proteins were produced by in vitro transcription-translation or in Escherichia coli and tested for their biological activity using the hybridoma growth factor (HGF) assay or a transcriptional activation assay on human hepatoma cells. The folding of the mutants was also checked by immunoprecipitation with conformation-specific monoclonal antibodies. The results show that only residues 29 to 34 are crucial for IL-6 activity and that the first two amino acids are probably involved in the definition of the IL-6 active site.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalFEBS Letters
Volume288
Issue number1-2
DOIs
Publication statusPublished - Aug 19 1991

Fingerprint

Bioactivity
Interleukin-6
Amino Acids
Assays
Mutant Proteins
Transcription
Immunoprecipitation
Escherichia coli
Transcriptional Activation
Conformations
Hepatocellular Carcinoma
Catalytic Domain
Complementary DNA
Chemical activation
Monoclonal Antibodies

Keywords

  • CRP/CAT assay
  • HGF assay
  • Interleukin-6
  • Internal deletions
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Cite this

Internal deletions of amino acids 29-42 of human interleukin-6 (IL-6) differentially affect bioactivity and folding. / Arcone, Rosaria; Fontaine, Veronique; Coto, Iolanda; Content, Jean; Ciliberto, Gennaro.

In: FEBS Letters, Vol. 288, No. 1-2, 19.08.1991, p. 197-200.

Research output: Contribution to journalArticle

Arcone, Rosaria ; Fontaine, Veronique ; Coto, Iolanda ; Content, Jean ; Ciliberto, Gennaro. / Internal deletions of amino acids 29-42 of human interleukin-6 (IL-6) differentially affect bioactivity and folding. In: FEBS Letters. 1991 ; Vol. 288, No. 1-2. pp. 197-200.
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