Internal deletions of amino acids 29-42 of human interleukin-6 (IL-6) differentially affect bioactivity and folding

Rosaria Arcone, Veronique Fontaine, Iolanda Coto, Jean Content, Gennaro Ciliberto

Research output: Contribution to journalArticle

Abstract

Internal deletions of the human interleukin-6 (IL-6) cDNA have been generated in the region encoding residues 29 to 42. Mutant proteins were produced by in vitro transcription-translation or in Escherichia coli and tested for their biological activity using the hybridoma growth factor (HGF) assay or a transcriptional activation assay on human hepatoma cells. The folding of the mutants was also checked by immunoprecipitation with conformation-specific monoclonal antibodies. The results show that only residues 29 to 34 are crucial for IL-6 activity and that the first two amino acids are probably involved in the definition of the IL-6 active site.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalFEBS Letters
Volume288
Issue number1-2
DOIs
Publication statusPublished - Aug 19 1991

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Keywords

  • CRP/CAT assay
  • HGF assay
  • Interleukin-6
  • Internal deletions
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

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