International study on inter-reader variability for circulating tumor cells in breast cancer

Michail Ignatiadis, Sabine Riethdorf, François Clement Bidard, Isabelle Vaucher, Mustapha Khazour, Françoise Rothé, Jessica Metallo, Ghizlane Rouas, Rachel E. Payne, Raoul C. Coombes, Ingrid Teufel, Ulrich Andergassen, Stella Apostolaki, Eleni Politaki, Dimitris Mavroudis, Silvia Bessi, Marta Pestrin, Angelo Di Leo, Michael Campion, Monica ReinholzEdith Perez, Martine Piccart, Elin Borgen, Bjorn Naume, Jose Jimenez, Claudia M. Aura, Laura Zorzino, Maria C. Cassatella, Maria T. Sandri, Bianca Mostert, Stefan Sleijfer, Jaco Kraan, Wolfgang Janni, Tanja Fehm, Brigitte Rack, Leon Terstappen, Madeline Repollet, Jean Yves Pierga, Craig Miller, Christos Sotiriou, Stefan Michiels, Klaus Pantel

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Abstract

Introduction: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.Methods: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.Results: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P <0.001) and from M0 and

Original languageEnglish
Article numberR43
JournalBreast Cancer Research
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 23 2014

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Circulating Neoplastic Cells
Breast Neoplasms
Cell Count
Nonparametric Statistics
Artifacts
Leukocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Ignatiadis, M., Riethdorf, S., Bidard, F. C., Vaucher, I., Khazour, M., Rothé, F., ... Pantel, K. (2014). International study on inter-reader variability for circulating tumor cells in breast cancer. Breast Cancer Research, 16(2), [R43]. https://doi.org/10.1186/bcr3647

International study on inter-reader variability for circulating tumor cells in breast cancer. / Ignatiadis, Michail; Riethdorf, Sabine; Bidard, François Clement; Vaucher, Isabelle; Khazour, Mustapha; Rothé, Françoise; Metallo, Jessica; Rouas, Ghizlane; Payne, Rachel E.; Coombes, Raoul C.; Teufel, Ingrid; Andergassen, Ulrich; Apostolaki, Stella; Politaki, Eleni; Mavroudis, Dimitris; Bessi, Silvia; Pestrin, Marta; Di Leo, Angelo; Campion, Michael; Reinholz, Monica; Perez, Edith; Piccart, Martine; Borgen, Elin; Naume, Bjorn; Jimenez, Jose; Aura, Claudia M.; Zorzino, Laura; Cassatella, Maria C.; Sandri, Maria T.; Mostert, Bianca; Sleijfer, Stefan; Kraan, Jaco; Janni, Wolfgang; Fehm, Tanja; Rack, Brigitte; Terstappen, Leon; Repollet, Madeline; Pierga, Jean Yves; Miller, Craig; Sotiriou, Christos; Michiels, Stefan; Pantel, Klaus.

In: Breast Cancer Research, Vol. 16, No. 2, R43, 23.04.2014.

Research output: Contribution to journalArticle

Ignatiadis, M, Riethdorf, S, Bidard, FC, Vaucher, I, Khazour, M, Rothé, F, Metallo, J, Rouas, G, Payne, RE, Coombes, RC, Teufel, I, Andergassen, U, Apostolaki, S, Politaki, E, Mavroudis, D, Bessi, S, Pestrin, M, Di Leo, A, Campion, M, Reinholz, M, Perez, E, Piccart, M, Borgen, E, Naume, B, Jimenez, J, Aura, CM, Zorzino, L, Cassatella, MC, Sandri, MT, Mostert, B, Sleijfer, S, Kraan, J, Janni, W, Fehm, T, Rack, B, Terstappen, L, Repollet, M, Pierga, JY, Miller, C, Sotiriou, C, Michiels, S & Pantel, K 2014, 'International study on inter-reader variability for circulating tumor cells in breast cancer', Breast Cancer Research, vol. 16, no. 2, R43. https://doi.org/10.1186/bcr3647
Ignatiadis M, Riethdorf S, Bidard FC, Vaucher I, Khazour M, Rothé F et al. International study on inter-reader variability for circulating tumor cells in breast cancer. Breast Cancer Research. 2014 Apr 23;16(2). R43. https://doi.org/10.1186/bcr3647
Ignatiadis, Michail ; Riethdorf, Sabine ; Bidard, François Clement ; Vaucher, Isabelle ; Khazour, Mustapha ; Rothé, Françoise ; Metallo, Jessica ; Rouas, Ghizlane ; Payne, Rachel E. ; Coombes, Raoul C. ; Teufel, Ingrid ; Andergassen, Ulrich ; Apostolaki, Stella ; Politaki, Eleni ; Mavroudis, Dimitris ; Bessi, Silvia ; Pestrin, Marta ; Di Leo, Angelo ; Campion, Michael ; Reinholz, Monica ; Perez, Edith ; Piccart, Martine ; Borgen, Elin ; Naume, Bjorn ; Jimenez, Jose ; Aura, Claudia M. ; Zorzino, Laura ; Cassatella, Maria C. ; Sandri, Maria T. ; Mostert, Bianca ; Sleijfer, Stefan ; Kraan, Jaco ; Janni, Wolfgang ; Fehm, Tanja ; Rack, Brigitte ; Terstappen, Leon ; Repollet, Madeline ; Pierga, Jean Yves ; Miller, Craig ; Sotiriou, Christos ; Michiels, Stefan ; Pantel, Klaus. / International study on inter-reader variability for circulating tumor cells in breast cancer. In: Breast Cancer Research. 2014 ; Vol. 16, No. 2.
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abstract = "Introduction: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch{\circledR} system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.Methods: CellSearch{\circledR} images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using {\%} agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.Results: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92{\%} (range 69 to 97{\%}) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91{\%}, range 70 to 96{\%}) compared to M1 (median 98{\%}, range 64 to 100{\%}) patients (P <0.001) and from M0 and",
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AU - Ignatiadis, Michail

AU - Riethdorf, Sabine

AU - Bidard, François Clement

AU - Vaucher, Isabelle

AU - Khazour, Mustapha

AU - Rothé, Françoise

AU - Metallo, Jessica

AU - Rouas, Ghizlane

AU - Payne, Rachel E.

AU - Coombes, Raoul C.

AU - Teufel, Ingrid

AU - Andergassen, Ulrich

AU - Apostolaki, Stella

AU - Politaki, Eleni

AU - Mavroudis, Dimitris

AU - Bessi, Silvia

AU - Pestrin, Marta

AU - Di Leo, Angelo

AU - Campion, Michael

AU - Reinholz, Monica

AU - Perez, Edith

AU - Piccart, Martine

AU - Borgen, Elin

AU - Naume, Bjorn

AU - Jimenez, Jose

AU - Aura, Claudia M.

AU - Zorzino, Laura

AU - Cassatella, Maria C.

AU - Sandri, Maria T.

AU - Mostert, Bianca

AU - Sleijfer, Stefan

AU - Kraan, Jaco

AU - Janni, Wolfgang

AU - Fehm, Tanja

AU - Rack, Brigitte

AU - Terstappen, Leon

AU - Repollet, Madeline

AU - Pierga, Jean Yves

AU - Miller, Craig

AU - Sotiriou, Christos

AU - Michiels, Stefan

AU - Pantel, Klaus

PY - 2014/4/23

Y1 - 2014/4/23

N2 - Introduction: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.Methods: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.Results: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P <0.001) and from M0 and

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