Abstract
The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin-and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABAB receptor-mediated Ca2+ elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits.
| Original language | English |
|---|---|
| Article number | 82 |
| Journal | Nature Communications |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 1 2018 |
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ASJC Scopus subject areas
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)
Cite this
Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes. / Mariotti, Letizia; Losi, Gabriele; Lia, Annamaria; Melone, Marcello; Chiavegato, Angela; Gómez-Gonzalo, Marta; Sessolo, Michele; Bovetti, Serena; Forli, Angelo; Zonta, Micaela; Requie, Linda Maria; Marcon, Iacopo; Pugliese, Arianna; Viollet, Cécile; Bettler, Bernhard; Fellin, Tommaso; Conti, Fiorenzo; Carmignoto, Giorgio.
In: Nature Communications, Vol. 9, No. 1, 82, 01.12.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interneuron-specific signaling evokes distinctive somatostatin-mediated responses in adult cortical astrocytes
AU - Mariotti, Letizia
AU - Losi, Gabriele
AU - Lia, Annamaria
AU - Melone, Marcello
AU - Chiavegato, Angela
AU - Gómez-Gonzalo, Marta
AU - Sessolo, Michele
AU - Bovetti, Serena
AU - Forli, Angelo
AU - Zonta, Micaela
AU - Requie, Linda Maria
AU - Marcon, Iacopo
AU - Pugliese, Arianna
AU - Viollet, Cécile
AU - Bettler, Bernhard
AU - Fellin, Tommaso
AU - Conti, Fiorenzo
AU - Carmignoto, Giorgio
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin-and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABAB receptor-mediated Ca2+ elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits.
AB - The signaling diversity of GABAergic interneurons to post-synaptic neurons is crucial to generate the functional heterogeneity that characterizes brain circuits. Whether this diversity applies to other brain cells, such as the glial cells astrocytes, remains unexplored. Using optogenetics and two-photon functional imaging in the adult mouse neocortex, we here reveal that parvalbumin-and somatostatin-expressing interneurons, two key interneuron classes in the brain, differentially signal to astrocytes inducing weak and robust GABAB receptor-mediated Ca2+ elevations, respectively. Furthermore, the astrocyte response depresses upon parvalbumin interneuron repetitive stimulations and potentiates upon somatostatin interneuron repetitive stimulations, revealing a distinguished astrocyte plasticity. Remarkably, the potentiated response crucially depends on the neuropeptide somatostatin, released by somatostatin interneurons, which activates somatostatin receptors at astrocytic processes. Our study unveils, in the living brain, a hitherto unidentified signaling specificity between interneuron subtypes and astrocytes opening a new perspective into the role of astrocytes as non-neuronal components of inhibitory circuits.
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UR - http://www.scopus.com/inward/citedby.url?scp=85042128139&partnerID=8YFLogxK
U2 - 10.1038/s41467-017-02642-6
DO - 10.1038/s41467-017-02642-6
M3 - Article
C2 - 29311610
AN - SCOPUS:85042128139
VL - 9
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 82
ER -