Interplay of platelet polymorphisms, risk factors and Von Willebrand factor, and flow-mediated conditions in determining collagen-adenosine diphosphate PFA-100 results in patients with coronary artery disease

Italo Porto, Antonio Maria Leone, Luisa Nanni, Alessandro Sciahbasi, Maria De Vita, Gaetano Antonio Lanza, Felicita Andreotti

Research output: Contribution to journalArticlepeer-review

Abstract

Platelets play a pivotal role in thrombus formation in patients with coronary artery disease (CAD), since the high shear generated in the presence of severe coronary stenoses can increase platelet reactivity (PR) and trigger thrombogenesis. Several reports have suggested a functional effect of human platelet antigen (HPA)-1 and HPA-2 gene polymorphisms on PR. However, the true determinants of high-shear PR in CAD patients taking their usual medications are still incompletely understood. In 104 patients with stable CAD we analyzed the possible clinical, biochemical and genetic factors affecting high-shear PR, measured by the ex vivo platelet function analyzer (PFA-100) collagen-adenosine diphosphate method. In univariate analysis, a lower PR was associated with decreased plasma von Willebrand factor-ristocetin cofactor activity, increased blood levels of triglycerides, female sex, use of thienopyridines, lower platelet count, and HPA-1b carriership. All variables, except HPA-1b, remained associated with lower PR in multivariate analysis. However, the introduction in the model of the HPA-1 and HPA-2 genotypes as interaction terms led to a significant improvement in the prediction of PR, although the quantitative effect was small (about 3% improvement, P = 0.046). Thus, in CAD patients, there seems to be only a mild effect of the platelet glycoprotein HPA-1 and HPA-2 polymorphisms on collagen-adenosine diphosphate-stimulated PR after the effect of well-established clinical and biochemical determinants are considered.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalBlood Coagulation and Fibrinolysis
Volume16
Issue number2
Publication statusPublished - Mar 2005

Keywords

  • Coronary artery disease
  • Genetic polymorphisms
  • Platelet function analyzer (PFA-100)
  • Platelet reactivity]

ASJC Scopus subject areas

  • Hematology

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