Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains

Montarop Yamabhai, Noah G. Hoffman, Nancy L. Hardison, Peter S. McPherson, Luisa Castagnoli, Gianni Cesareni, Brian K. Kay

Research output: Contribution to journalArticle

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Abstract

We screened a Xenopus laevis oocyte cDNA expression library with a Src homology 3 (SH3) class II peptide ligand and identified a 1270-amino acid- long protein containing two Eps15 homology (EH) domains, a central coiled- coil region, and five SH3 domains. We named this protein Intersectin, because it potentially brings together EH and SH3 domain-binding proteins into a macromolecular complex. The ligand preference of the EH domains were deduced to be asparajine-proline-phenylalanine (NPF) or cyclized NPF (CX(1- 2)NPFXXC), depending on the type of phage-displayed combinatorial peptide library used. Screens of a mouse embryo cDNA library with the EH domains of Intersectin yielded clones for the Rev-associated binding/Rev-interacting protein (RAB/Rip) and two novel proteins, which we named Intersectin-binding proteins (Ibps) 1 and 2. All three proteins contain internal and C-terminal NPF peptide sequences, and Ibp1 and Ibp2 also contain putative clathrin- binding sites. Deletion of the C-terminal sequence, NPFL-COOH, from RAB/Rip eliminated EH domain binding, whereas fusion of the same peptide sequence to glutathione S-transferase generated strong binding to the EH domains of Intersectin. Several experiments support the conclusion that the free carboxylate group contributes to binding of the NPFL motif at the C terminus of RAB/Rip to the EH domains of Intersectin. Finally, affinity selection experiments with the SH3 domains of Intersectin identified two endocytic proteins, dynamin and synaptojanin, as potential interacting proteins. We propose that Intersectin is a component of the endocytic machinery.

Original languageEnglish
Pages (from-to)31401-31407
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number47
DOIs
Publication statusPublished - Nov 20 1998

Fingerprint

src Homology Domains
rev Gene Products
Proteins
Gene Library
Peptides
Carrier Proteins
Ligands
Dynamins
Macromolecular Substances
Peptide Library
Clathrin
Xenopus laevis
intersectin 1
Glutathione Transferase
Phenylalanine
Proline
Bacteriophages
Oocytes
Embryonic Structures
Clone Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yamabhai, M., Hoffman, N. G., Hardison, N. L., McPherson, P. S., Castagnoli, L., Cesareni, G., & Kay, B. K. (1998). Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains. Journal of Biological Chemistry, 273(47), 31401-31407. https://doi.org/10.1074/jbc.273.47.31401

Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains. / Yamabhai, Montarop; Hoffman, Noah G.; Hardison, Nancy L.; McPherson, Peter S.; Castagnoli, Luisa; Cesareni, Gianni; Kay, Brian K.

In: Journal of Biological Chemistry, Vol. 273, No. 47, 20.11.1998, p. 31401-31407.

Research output: Contribution to journalArticle

Yamabhai, M, Hoffman, NG, Hardison, NL, McPherson, PS, Castagnoli, L, Cesareni, G & Kay, BK 1998, 'Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains', Journal of Biological Chemistry, vol. 273, no. 47, pp. 31401-31407. https://doi.org/10.1074/jbc.273.47.31401
Yamabhai, Montarop ; Hoffman, Noah G. ; Hardison, Nancy L. ; McPherson, Peter S. ; Castagnoli, Luisa ; Cesareni, Gianni ; Kay, Brian K. / Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 47. pp. 31401-31407.
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