Abstract
Original language | English |
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Pages (from-to) | 156-170 |
Number of pages | 15 |
Journal | Eur. Cells and Mater. |
Volume | 39 |
DOIs | |
Publication status | Published - 2020 |
Keywords
- Annulus fibrosus
- Endplate
- Intervertebral disc
- Microarray
- Nucleus pulposus
- Phenotype
- adipogenesis
- adult
- annulus fibrosus
- Article
- bone development
- cell aging
- cell differentiation
- cell expansion
- cell function
- cell isolation
- cell population
- cell proliferation
- cell therapy
- clinical article
- clonogenesis
- controlled study
- female
- gene expression profiling
- human
- human cell
- human tissue
- immunophenotyping
- in vitro study
- intervertebral disk degeneration
- male
- microarray analysis
- middle aged
- nerve ending
- nucleus pulposus
- RNA isolation
- surgical technique
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Intervertebral disc and endplate cell characterisation highlights annulus fibrosus cells as the most promising for tissue-specific disc degeneration therapy : European Cells and Materials. / De Luca, P.; Castagnetta, M.; de Girolamo, L.; Coco, S.; Malacarne, M.; Ragni, E.; Viganò, M.; Lugano, G.; Brayda-Bruno, M.; Coviello, D.; Colombini, A.
In: Eur. Cells and Mater., Vol. 39, 2020, p. 156-170.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Intervertebral disc and endplate cell characterisation highlights annulus fibrosus cells as the most promising for tissue-specific disc degeneration therapy
T2 - European Cells and Materials
AU - De Luca, P.
AU - Castagnetta, M.
AU - de Girolamo, L.
AU - Coco, S.
AU - Malacarne, M.
AU - Ragni, E.
AU - Viganò, M.
AU - Lugano, G.
AU - Brayda-Bruno, M.
AU - Coviello, D.
AU - Colombini, A.
N1 - Cited By :2 Export Date: 24 February 2021 Correspondence Address: de Girolamo, L.via Riccardo Galeazzi 4, Italy; email: laura.degirolamo@grupposandonato.it Funding text 1: The study was funded by the Italian Ministry of Funding text 2: Health, “Ricerca Corrente”. S.C. is a PhD supported by grants from the Italian Ministry of Health (Ricerca Corrente). The authors declare no conflict of interest. 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The International Society for Cellular Therapy position statement (2006) Cytotherapy, 8, pp. 315-317; Gruber, H.E., Riley, F.E., Hoelscher, G.L., Ingram, J.A., Bullock, L., Hanley, E.N., Jr., Human annulus progenitor cells: Analyses of this viable endogenous cell population (2016) J Orthop Res, 34, pp. 1351-1360; Huang, B., Liu, L.T., Li, C.Q., Zhuang, Y., Luo, G., Hu, S.Y., Zhou, Y., Study to determine the presence of progenitor cells in the degenerated human cartilage endplates (2012) Eur Spine J, 21, pp. 613-622; Jackson, A.R., Huang, C.Y., Gu, W.Y., Effect of endplate calcification and mechanical deformation on the distribution of glucose in intervertebral disc: A 3D finite element study (2011) Comput Methods Biomech Biomed Engin, 14, pp. 195-204; Keyes, W.M., Mills, A.A., P63: A new link between senescence and aging (2006) Cell Cycle, 5, pp. 260-265; Lee, H.J., Choi, B.H., Min, B.H., Park, S.R., Changes in surface markers of human mesenchymal stem cells during the chondrogenic differentiation and dedifferentiation processes vo (2009) Arthritis Rheum, 60, pp. 2325-2332; 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PY - 2020
Y1 - 2020
N2 - Degenerative processes of the intervertebral disc (IVD) and cartilaginous endplate lead to chronic spine pathologies. Several studies speculated on the intrinsic regenerative capacity of degenerated IVD related to the presence of local mesenchymal progenitors. However, a complete characterisation of the resident IVD cell populations, particularly that isolated from the endplate, is lacking. The purpose of the present study was to characterise the gene expression profiles of human nucleus pulposus (NPCs), annulus fibrosus (AFCs) and endplate (EPCs) cells, setting the basis for future studies aimed at identifying the most promising cells for regenerative purposes. Cells isolated from NP, AF and EP were analysed after vo expansion for their stemness ability, immunophenotype and gene profiles by large-scale microarray analysis. The three cell populations shared a similar clonogenic, adipogenic and osteogenic potential, as well as an immunophenotype with a pattern resembling that of mesenchymal stem cells. NPCs maintained the greatest chondrogenic potential and shared with EPCs the loss of proliferation capability during expansion. The largest number of selectively highly expressed stemness, chondrogenic/tissue-specific and surface genes was found in AFCs, thus representing the most promising source of tissue-specific expanded cells for the treatment of IVD degeneration. © 2020, AO Research Institute Davos. All rights reserved.
AB - Degenerative processes of the intervertebral disc (IVD) and cartilaginous endplate lead to chronic spine pathologies. Several studies speculated on the intrinsic regenerative capacity of degenerated IVD related to the presence of local mesenchymal progenitors. However, a complete characterisation of the resident IVD cell populations, particularly that isolated from the endplate, is lacking. The purpose of the present study was to characterise the gene expression profiles of human nucleus pulposus (NPCs), annulus fibrosus (AFCs) and endplate (EPCs) cells, setting the basis for future studies aimed at identifying the most promising cells for regenerative purposes. Cells isolated from NP, AF and EP were analysed after vo expansion for their stemness ability, immunophenotype and gene profiles by large-scale microarray analysis. The three cell populations shared a similar clonogenic, adipogenic and osteogenic potential, as well as an immunophenotype with a pattern resembling that of mesenchymal stem cells. NPCs maintained the greatest chondrogenic potential and shared with EPCs the loss of proliferation capability during expansion. The largest number of selectively highly expressed stemness, chondrogenic/tissue-specific and surface genes was found in AFCs, thus representing the most promising source of tissue-specific expanded cells for the treatment of IVD degeneration. © 2020, AO Research Institute Davos. All rights reserved.
KW - Annulus fibrosus
KW - Endplate
KW - Intervertebral disc
KW - Microarray
KW - Nucleus pulposus
KW - Phenotype
KW - adipogenesis
KW - adult
KW - annulus fibrosus
KW - Article
KW - bone development
KW - cell aging
KW - cell differentiation
KW - cell expansion
KW - cell function
KW - cell isolation
KW - cell population
KW - cell proliferation
KW - cell therapy
KW - clinical article
KW - clonogenesis
KW - controlled study
KW - female
KW - gene expression profiling
KW - human
KW - human cell
KW - human tissue
KW - immunophenotyping
KW - in vitro study
KW - intervertebral disk degeneration
KW - male
KW - microarray analysis
KW - middle aged
KW - nerve ending
KW - nucleus pulposus
KW - RNA isolation
KW - surgical technique
U2 - 10.22203/eCM.v039a10
DO - 10.22203/eCM.v039a10
M3 - Article
VL - 39
SP - 156
EP - 170
JO - Eur. Cells and Mater.
JF - Eur. Cells and Mater.
SN - 1473-2262
ER -