Intestinal epithelial cells control dendritic cell function

Monica Rimoldi, Marcello Chieppa, Marisa Vulcano, Paola Allavena, Maria Rescigno

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Dendritic cells (DCs) comprise a family of cells specializing in antigen capture and presentation to T cells. We have recently shown that DC play an active role in bacterial uptake across mucosal surfaces. Indeed, DC are able to open tight junctions and to sample antigens directly across epithelia, both in vitro and in vivo. Because DC express tight junction proteins, the integrity of the epithelial barrier is preserved. In this study we have analyzed the possible involvement of epithelial cells in controlling DC function. We developed an in vitro model in our laboratory consisting of a three-player system of dendritic cells, epithelial cell monolayers, and bacteria. The crosstalk between epithelial cells and dendritic cells was analyzed, and epithelial cells were tested for their capacity to release cytokines and chemokines that induce the migration and activation of DC. We show that the capacity of epithelial eels to produce cytokines and activate DC is dependent on the invasiveness of the bacteria tested. In particular, invasive bacteria stimulate epithelial cells to release proinflammatory cytokines and to induce the maturation state of DC. By contrast, noninvasive bacteria are unable to stimulate epithelial cells, but can activate DC directly when DC translocate to the apical side. In conclusion, epithelial cells are not simply a barrier to bacteria entering via the oral route, but actively influence the activating properties of bystander DC.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume1029
DOIs
Publication statusPublished - 2004

Fingerprint

Dendritic Cells
Epithelial Cells
Bacteria
Cytokines
Cells
Tight Junction Proteins
Antigens
Eels
T-cells
Tight Junctions
Antigen Presentation
Crosstalk
Chemokines
Monolayers
Epithelium
Chemical activation
T-Lymphocytes

Keywords

  • Bacteria
  • Dendritic cells
  • Epithelial cells
  • Intestine
  • JAM
  • Mucosa
  • Tight junctions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Intestinal epithelial cells control dendritic cell function. / Rimoldi, Monica; Chieppa, Marcello; Vulcano, Marisa; Allavena, Paola; Rescigno, Maria.

In: Annals of the New York Academy of Sciences, Vol. 1029, 2004, p. 66-74.

Research output: Contribution to journalArticle

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