Intoxication caused by new psychostimulants

analytical methods to disclose acute and chronic use of benzofurans and ethylphenidate

Bernardino Barceló, Isabel Gomila, Maria Concetta Rotolo, Emilia Marchei, Chrystalla Kyriakou, Simona Pichini, Carolina Roset, Miguel Ángel Elorza, Francesco Paolo Busardò

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The acute and chronic toxicity of several new psychoactive substances (NPS) is unknown, and only little information is available on the pharmacology and toxicology, toxicokinetics, and detectability in body samples of such new compounds. We here propose analytical methods to disclose acute and chronic use of two types of new psychostimulants: benzofurans and ethylphenidate and we applied them to a real case of a subject attending Emergency Department with signs of acute intoxication due to psychotropic drug(s). After a urinary immunoassay screening which gave a positivity to amphetamines, general unknown gas chromatography–mass spectrometry (GC-MS) urine analysis identified 5-(2-methylaminopropyl)benzofuran (5-MAPB), 5-(2-aminopropyl)benzofuran (5-APB), 5-(2-ethylaminopropyl)benzofuran (5-EAPB), ethylphenidate, and ritalinic acid. All these substances were confirmed and quantified not only in urine but also in serum samples at different times after hospitalization by GC-MS and ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Two subsequent 2-cm hair segments were also analyzed and tested positive for the above reported substances, evidencing repeated use. The matching quantitative results in all the analyzed biological matrices demonstrated that both analytical methodologies were suitable to correctly quantify NPS involved in the current intoxication. The objective assessment of acute and chronic intoxication by the above reported compounds demonstrate that the development of analytical methods aiming at the detection of a broad spectrum of compounds in conventional and non-conventional biological matrices is helpful when facing the new challenging threat of intoxications caused by NPS.

Original languageEnglish
Pages (from-to)1543-1553
Number of pages11
JournalInternational Journal of Legal Medicine
Volume131
Issue number6
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

ethylphenidate
Benzofurans
ritalinic acid
Spectrum Analysis
Gases
Urine
Amphetamines
Psychotropic Drugs
Tandem Mass Spectrometry
Immunoassay
Toxicology
Hospital Emergency Service
Hospitalization
High Pressure Liquid Chromatography
Pharmacology
Serum
benzofuran

Keywords

  • Benzofurans
  • Ethylphenidate
  • GC-MS
  • Intoxication
  • UHPLC-MS/MS

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Intoxication caused by new psychostimulants : analytical methods to disclose acute and chronic use of benzofurans and ethylphenidate. / Barceló, Bernardino; Gomila, Isabel; Rotolo, Maria Concetta; Marchei, Emilia; Kyriakou, Chrystalla; Pichini, Simona; Roset, Carolina; Elorza, Miguel Ángel; Busardò, Francesco Paolo.

In: International Journal of Legal Medicine, Vol. 131, No. 6, 01.11.2017, p. 1543-1553.

Research output: Contribution to journalArticle

Barceló, Bernardino ; Gomila, Isabel ; Rotolo, Maria Concetta ; Marchei, Emilia ; Kyriakou, Chrystalla ; Pichini, Simona ; Roset, Carolina ; Elorza, Miguel Ángel ; Busardò, Francesco Paolo. / Intoxication caused by new psychostimulants : analytical methods to disclose acute and chronic use of benzofurans and ethylphenidate. In: International Journal of Legal Medicine. 2017 ; Vol. 131, No. 6. pp. 1543-1553.
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AU - Marchei, Emilia

AU - Kyriakou, Chrystalla

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AU - Roset, Carolina

AU - Elorza, Miguel Ángel

AU - Busardò, Francesco Paolo

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