Intra-bone marrow injection of bone marrow and cord blood cells: An alternative way of transplantation associated with a higher seeding efficiency

Sandra Castello, Marina Podestà, Vincenzo G. Menditto, Adalberto Ibatici, Anna Pitto, Osvaldo Figari, Daniele Scarpati, Lorenzo Magrassi, Andrea Bacigalupo, Giovanna Piaggio, Francesco Frassoni

Research output: Contribution to journalArticlepeer-review

Abstract

Intravenous (IV) injection is currently the normal method for transplanting hematopoietic cells. However, the problem of seeding efficiency and homing is relevant especially when a limited number of stem cells is available. Intra-bone marrow (IBM) injection of bone marrow cells (BMCs) may overcome this problem.Irradiated (750 cGy) C57BL/6J mice were transplanted with 1 × 10 5 BMCs harvested from transgenic mice expressing an enhanced version of the green fluorescent protein (EGFP +) via IBM or with 1 × 10 6 EGFP + BMCs via IV. Irradiated (320 cGy) NOD/SCID mice were transplanted with 1 × 10 6 human cord blood (CB) cells via IBM or with 1 × 10 7 human CB cells via IV.In C57BL/6J mice after 90 days, the fraction of EGFP + cells harvested was 37% and 53% in IV-treated and IBM-treated (contralateral tibia and femur in the IBM) mice, respectively: the expansion folds were 114 and 1760, respectively. In NOD/SCID mice, the percentages of CD45 + cells and CD45 +/CD34 + cells were, at 30 days, 3.3% and 0.3% in IV-treated mice, and 4.4% and 1.1% in IBM-treated mice. At 60 days, the percentages of CD45 + cells and CD45 +/CD34 + cells were 2.1% and 0.3% in IV-treated mice and 1.4% and 0.4% in IBM-treated mice. At day 90 the percentages of CD45 + cells and CD45 +/CD34 + cells were 3% and 0.3% in IV-treated mice and 2.3% and 0.4% in IBM-treated mice.Our data demonstrate that IBM transplantation is associated with a seeding efficiency 15 times greater than IV transplantation. IBM transplantation may improve the results of transplant and may be useful in several settings: 1) when a limited number of hematopoietic progenitors are available; and 2) in experiments aiming to place in the bone marrow stem cells of other lineages (CNS, muscle, etc.).

Original languageEnglish
Pages (from-to)782-787
Number of pages6
JournalExperimental Hematology
Volume32
Issue number8
DOIs
Publication statusPublished - Aug 2004

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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