Intracellular association of FGF-2 with the ribosomal protein LG/TAXREB107

Bin Shen, Marco Arese, Anna Gualandris, Daniel B. Rifkin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

By using the yeast two-hybrid system, we identified the ribosomal protein L6/TAXREB107 as an intracellular partner for FGF-2. L6/TAXREB107 also mediates the DNA binding of the HTLV-1 transactivator Tax. In vitro binding experiments indicated that both the high-molecular-weight forms (HMW) and the 18-kDa form of FGF-2 bind to L6/TAXREB107. Deletion analysis suggested that L6/TAXREB107 has two binding sites for HMW FGF-2 and one binding site for 18-kDa FGF-2, implying that the unique N-terminal extension of the HMW FGF-2 is one of the binding domains for L6/TAXREB107. Transfection assays showed that high expression of either HMW or 18 kDa FGF-2 stimulates Tax-mediated transactivation in NIH 3T3 cells. This result suggests a possible role of FGF-2 in Tax-mediated HTLV-1 transformation as well as FGF-2 binding to ribosomes and/or their precursors.

Original languageEnglish
Pages (from-to)524-528
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number2
DOIs
Publication statusPublished - Nov 18 1998

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Ribosomal Proteins
Fibroblast Growth Factor 2
Association reactions
Taxation
Molecular Weight
Molecular weight
Human T-lymphotropic virus 1
Binding Sites
NIH 3T3 Cells
Two-Hybrid System Techniques
Trans-Activators
mouse tax-responsive-element-binding protein 107
Hybrid systems
Ribosomes
Yeast
Transcriptional Activation
Transfection
Assays
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Intracellular association of FGF-2 with the ribosomal protein LG/TAXREB107. / Shen, Bin; Arese, Marco; Gualandris, Anna; Rifkin, Daniel B.

In: Biochemical and Biophysical Research Communications, Vol. 252, No. 2, 18.11.1998, p. 524-528.

Research output: Contribution to journalArticle

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