Intracellular detection of laminin α2 chain in skin by electron microscopy immunocytochemistry

Comparison between normal and laminin α2 chain deficient subjects

S. Squarzoni, M. Villanova, P. Sabatelli, A. Malandrini, P. Toti, A. Pini, L. Merlini, G. C. Guazzi, N. M. Maraldi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of this study is to localize the α2 laminin chain in normal human skin. The methods used were immune-gold cytochemistry on cryo-ultramicrotomy sections and thin-section-fracture-label, together with electron microscopy observation. Results were compared with light microscopy peroxidase immune-staining. Both normal skin samples and skin biopsies from laminin α2 chain deficient congenital muscular dystrophy affected patients were studied. The results show that, in normal skin, the laminin α2 chain is spread throughout the cytoplasm of basal keratinocytes, while it appears associated with desmosomal tonofilaments in the spinous and granular epidermal layers; in skin samples from dystrophic patients the laminin α2 chain was not detectable, These data suggest that the function of the laminin α2 chain is different in the epidermis as compared to that in muscle and peripheral nerve, where it is localized in the basement membrane.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalNeuromuscular Disorders
Volume7
Issue number2
DOIs
Publication statusPublished - Mar 1997

Fingerprint

Laminin
Electron Microscopy
Immunohistochemistry
Skin
Microtomy
Histocytochemistry
Intermediate Filaments
Muscular Dystrophies
Keratinocytes
Peripheral Nerves
Basement Membrane
Epidermis
Gold
Peroxidase
Microscopy
Cytoplasm
Observation
Staining and Labeling
Biopsy
Light

Keywords

  • Cytoskeleton
  • Electron microscopy immunocytochemistry
  • Extracellular matrix
  • Laminin 2
  • Signalling
  • Thin section fracture label

ASJC Scopus subject areas

  • Clinical Neurology
  • Pediatrics, Perinatology, and Child Health
  • Developmental Neuroscience
  • Neurology

Cite this

Intracellular detection of laminin α2 chain in skin by electron microscopy immunocytochemistry : Comparison between normal and laminin α2 chain deficient subjects. / Squarzoni, S.; Villanova, M.; Sabatelli, P.; Malandrini, A.; Toti, P.; Pini, A.; Merlini, L.; Guazzi, G. C.; Maraldi, N. M.

In: Neuromuscular Disorders, Vol. 7, No. 2, 03.1997, p. 91-98.

Research output: Contribution to journalArticle

Squarzoni, S. ; Villanova, M. ; Sabatelli, P. ; Malandrini, A. ; Toti, P. ; Pini, A. ; Merlini, L. ; Guazzi, G. C. ; Maraldi, N. M. / Intracellular detection of laminin α2 chain in skin by electron microscopy immunocytochemistry : Comparison between normal and laminin α2 chain deficient subjects. In: Neuromuscular Disorders. 1997 ; Vol. 7, No. 2. pp. 91-98.
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