Intracellular expression of P-170 glycoprotein in peripheral blood mononuclear cell subsets from healthy donors and HIV-infected patients

Walter Malorni, Mothanjie Barbara Lucia, Gabriella Rainaldi, Roberto Cauda, Maurizio Cianfriglia, Gianfranco Donelli, Luigi Ortona

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Abstract

Background and Objective. P-glycoprotein (P-gp) is a transmembrane efflux pump that actively extrude a variety of unrelated drugs from cancer cells, leading to the so-called multidrug resistance (MDR) phenomenon. However, P-gp has also been found in normal bone marrow and peripheral blood mononuclear cells (PBMC). Recently, the presence of P-glycoprotein in PBMC from human immunodeficiency virus (HIV)-infected patients has also been investigated and e phenotype-associated P-gp expression has been detected. Design and Methods. A total of thirty-eight HIV-1 positive patients with a mean age of 34 years (range, 24-41 years) were studied after an informed consent. Peripheral blood mononuclear cells (PBMC) were isolated by centrifugation on a Ficoll/Hypaque and P-glycoprotein expression was investigated on lymphocyte population by single and double-color immunofluorescence techniques. We investigated: I) both surface and intracellular expression of the P-gp molecule in different PBMC subsets, II) P-gp expression modifications occurring during HIV infection, and III) the effect of HIV-gp120 on the expression of P-gp by T lymphocyte subsets from healthy donors. Results. Our experimental findings indicate that: a) P-gp glycoprotein can be detected on an intracellular level in different PBMC subpopulations (mainly CD8+ T lymphocytes, CD16+ NK cells and CD14+ monocytes); b) this intracellular expression is decreased in specific PBMC subsets (i.e. T-CD8+ and NK-CD16+) from HIV-infected patients and c) a rearrangement was obtained when CD4+ and CD8+ from healthy donors were exposed in vitro to the HIV-binding glycoprotein gp120. Interpretation and Conclusions. Our results indicate that P-gp glycoprotein can also be expressed intracellularity and can be rearranged in PBMC subsets from HIV- infected patients.

Original languageEnglish
Pages (from-to)13-20
Number of pages8
JournalHaematologica
Volume83
Issue number1
Publication statusPublished - Jan 1998

Fingerprint

P-Glycoprotein
Blood Cells
Tissue Donors
HIV
Glycoproteins
Diatrizoate
Virus Attachment
Ficoll
Multiple Drug Resistance
T-Lymphocyte Subsets
Virus Diseases
Informed Consent
Centrifugation
Natural Killer Cells
Fluorescent Antibody Technique
HIV-1
Monocytes
Color
Bone Marrow
Lymphocytes

Keywords

  • AIDS
  • HIV
  • Leukocyte
  • Multidrug resistance
  • P-glycoprotein

ASJC Scopus subject areas

  • Hematology

Cite this

Malorni, W., Lucia, M. B., Rainaldi, G., Cauda, R., Cianfriglia, M., Donelli, G., & Ortona, L. (1998). Intracellular expression of P-170 glycoprotein in peripheral blood mononuclear cell subsets from healthy donors and HIV-infected patients. Haematologica, 83(1), 13-20.

Intracellular expression of P-170 glycoprotein in peripheral blood mononuclear cell subsets from healthy donors and HIV-infected patients. / Malorni, Walter; Lucia, Mothanjie Barbara; Rainaldi, Gabriella; Cauda, Roberto; Cianfriglia, Maurizio; Donelli, Gianfranco; Ortona, Luigi.

In: Haematologica, Vol. 83, No. 1, 01.1998, p. 13-20.

Research output: Contribution to journalArticle

Malorni, W, Lucia, MB, Rainaldi, G, Cauda, R, Cianfriglia, M, Donelli, G & Ortona, L 1998, 'Intracellular expression of P-170 glycoprotein in peripheral blood mononuclear cell subsets from healthy donors and HIV-infected patients', Haematologica, vol. 83, no. 1, pp. 13-20.
Malorni, Walter ; Lucia, Mothanjie Barbara ; Rainaldi, Gabriella ; Cauda, Roberto ; Cianfriglia, Maurizio ; Donelli, Gianfranco ; Ortona, Luigi. / Intracellular expression of P-170 glycoprotein in peripheral blood mononuclear cell subsets from healthy donors and HIV-infected patients. In: Haematologica. 1998 ; Vol. 83, No. 1. pp. 13-20.
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